Astragalin from <i>Thesium chinense</i>: A Novel Anti-Aging and Antioxidant Agent Targeting IGFR/CD38/<i>Sirtuins</i>

• Main Authors: Ruifeng Wang (Author), Anping Ding (Author), Jiaye Wang (Author), Jiaxue Wang (Author), Yujie Zhou (Author), Miao Chen (Author), Shuang Ju (Author), Mingpu Tan (Author), Zengxu Xiang (Author)
• Format: Book
• Published: MDPI AG, 2024-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox13070859; 2076-3921
• Keywords: astragalin; zebrafish embryos; oxidative stress; <i>C. elegans</i>; antioxidant; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 13, Iss 7, p 859 (2024)

Astragalin (AG), a typical flavonoid found in <i>Thesium chinense</i> Turcz (<i>T. chinense</i>), is abundant in various edible plants and possesses high nutritional value, as well as antioxidant and antibacterial effects. In this study, we initially predicted the mechanism of action of AG with two anti-aging and antioxidant-related protein targets (CD38 and IGFR) by molecular docking and molecular dynamics simulation techniques. Subsequently, we examined the anti-aging effects of AG in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>), the antioxidant effects in zebrafish, and verified the related molecular mechanisms. In <i>C. elegans</i>, AG synergistically extended the lifespan of <i>C. elegans</i> by up-regulating the expression of <i>daf-16</i> through inhibiting the expression of <i>daf-2/IGFR</i> and also activating the AMPK and MAPK pathways to up-regulate the expression of <i>sir-2.1</i>, <i>sir-2.4</i>, and <i>skn-1</i>. In oxidatively damaged zebrafish embryos, AG demonstrated a synergistic effect in augmenting the resistance of zebrafish embryos to oxidative stress by up-regulating the expression levels of <i>SIRT1</i> and <i>SIRT6</i> within the zebrafish embryos system via the suppression of CD38 enzymatic activity and then inhibiting the expression of <i>IGFR</i> through high levels of <i>SIRT6</i>. These findings highlight the antioxidant and anti-aging properties of AG and indicate its potential application as a supplementary ingredient in aquaculture for enhancing fish health and growth.