Biochemical composition and antioxidant capacity of extracts from <it>Podophyllum hexandrum</it> rhizome

• Main Authors: Li Mengfei (Author), Zhou Lanlan (Author), Yang Delong (Author), Li Tiantian (Author), Li Wei (Author)
• Format: Book
• Published: BMC, 2012-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.1186/1472-6882-12-263; 1472-6882
• Keywords: <it>Podophyllum hexandrum</it>; Biochemical composition; Antioxidant capacity; GC-MS; Other systems of medicine; RZ201-999
• Source: BMC Complementary and Alternative Medicine, Vol 12, Iss 1, p 263 (2012)

<p>Abstract</p> <p>Background</p> <p><it>Podophyllum hexandrum</it> Royle (<it>P</it>. <it>hexandrum</it>) is a perennial herb and widely used in clinic. The present study was designed to separate and identify the biochemical composition and antioxidant capacity of extracts from <it>P</it>. <it>hexandrum</it> rhizome.</p> <p>Methods</p> <p>The ethyl acetate and ethanol extracts from <it>P</it>. <it>hexandrum</it> rhizome were analyzed by GC-MS (gas chromatography-mass spectrometry), and the antioxidant capacity of the extracts and the components was tested by using the DPPH (2, 2-diphenylpicrylhydrazyl) and FRAP (Ferric reducing/antioxidant power) assays.</p> <p>Results</p> <p>The rhizome extracts had greater antioxidant capacity than the petiole extracts in DPPH and FRAP assays. About 16 kinds of main reactive oxygen components were identified in the extracts. Components of PADE (Phthalic acid, diisobutyl ester), BADE (1,2-Benzenedicarboxylic acid, diisooctyl ester), Polyneuridine, PODD (Podophyllotoxin, deoxy), β-Sitosterol and POD (Podophyllotoxin) showed the antioxidant capacity in some degree. PODD, POD, and Polyneuridine showed stronger antioxidant capacity with the IC₅₀ and FRAP values of 9.61 ± 0.81 and 2923.98 ± 21.89 μM, 9.98 ± 0.24 and 2847.27 ± 14.82 μM, and 13.37 ± 0.35 and 2404.32 ± 36.88 μM, respectively, than the positive control ASA (Ascorbic acid) with the values of 60.78 ± 1.22 and 1267.5 ± 30.24 μM (<it>P</it> < 0.01).</p> <p>Conclusions</p> <p>PODD, POD, and Polyneuridine are very critical for the antioxidant capacity in the extract of <it>P</it>. <it>hexandrum</it> rhizome. These results provide useful biochemical basis and information for the potential use of this plant.</p>