Protective effect of <it>Launaea procumbens</it> (L.) on lungs against CCl₄-induced pulmonary damages in rat

• Main Author: Khan Rahmat (Author)
• Format: Book
• Published: BMC, 2012-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p><it>Launaea procumbens</it> (L.) is traditionally used in the treatment of various human ailments including pulmonary damages. The present study was arranged to evaluate the role of <it>Launaea procumbens</it> methanol extract (LME) against carbon tetrachloride (CCl₄) induced oxidative pulmonary damages in rat.</p> <p>Methods</p> <p>36 Sprague-Dawley male rats (170-180 g) were randomly divided into 06 groups. After a week of acclamization, group I was remained untreated while group II was given olive oil intraperitoneally (i.p.) and dimethyl sulfoxide <b>(</b>DMSO) orally, groups III, IV, V and VI were administered CCl₄, 3 ml/kg body weight (30% in olive oil i.p.). Groups IV, V were treated with 100 mg/kg, 200 mg/kg of LME whereas group VI was administered with 50 mg/kg body weight of rutin (RT) after 48 h of CCl₄ treatment for four weeks. Antioxidant profile in lungs were evaluated by estimating the activities of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GSR), glutathione peroxidase (GSH-Px), quinone reductase (QR) and reduced glutathione (GSH). CCl₄-induced lipid peroxidation was determined by measuring the level of thiobarbituric acid reactive substances (TBARS) with conjugation of deoxyribonucleic acid (DNA) damages, argyrophilic nucleolar organizer regions (AgNORs) counts and histopathology.</p> <p>Results</p> <p>Administration of CCl₄ for 6 weeks significantly <it>(p < 0.01)</it> reduced the activities of antioxidant enzymes and GSH concentration while increased TBARS contents and DNA damages in lung samples. Co-treatment of LME and rutin restored the activities of antioxidant enzymes and GSH contents. Changes in TBARS concentration and DNA fragmentation were significantly <it>(p < 0.01)</it> decreased with the treatment of LME and rutin in lung. Changes induced with CCl₄ in histopathology of lungs were significantly reduced with co-treatment of LME and rutin.</p> <p>Conclusion</p> <p>Results of present study revealed that LME could protect the lung tissues against CCl₄-induced oxidative stress possibly by improving the antioxidant defence system.</p>

Item Description

10.1186/1472-6882-12-133
1472-6882