Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation

Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively...

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Main Authors: Youzhen Wei (Author), Kun Wang (Author), Yafang Zhang (Author), Yi Duan (Author), Yan Tian (Author), Hongling Yin (Author), Xuelian Fu (Author), Zuan Ma (Author), Jianjun Zhou (Author), Min Yu (Author), Qingbin Ni (Author), Wenjie Tang (Author)
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Published: Frontiers Media S.A., 2023-03-01T00:00:00Z.
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100 1 0 |a Youzhen Wei  |e author 
700 1 0 |a Youzhen Wei  |e author 
700 1 0 |a Youzhen Wei  |e author 
700 1 0 |a Kun Wang  |e author 
700 1 0 |a Yafang Zhang  |e author 
700 1 0 |a Yi Duan  |e author 
700 1 0 |a Yan Tian  |e author 
700 1 0 |a Hongling Yin  |e author 
700 1 0 |a Xuelian Fu  |e author 
700 1 0 |a Zuan Ma  |e author 
700 1 0 |a Jianjun Zhou  |e author 
700 1 0 |a Min Yu  |e author 
700 1 0 |a Qingbin Ni  |e author 
700 1 0 |a Wenjie Tang  |e author 
700 1 0 |a Wenjie Tang  |e author 
245 0 0 |a Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation 
260 |b Frontiers Media S.A.,   |c 2023-03-01T00:00:00Z. 
500 |a 1663-9812 
500 |a 10.3389/fphar.2023.1138762 
520 |a Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol. 
546 |a EN 
690 |a hydrogen 
690 |a HIF-1α 
690 |a IL-1α 
690 |a chemokine 
690 |a inflammation 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n Frontiers in Pharmacology, Vol 14 (2023) 
787 0 |n https://www.frontiersin.org/articles/10.3389/fphar.2023.1138762/full 
787 0 |n https://doaj.org/toc/1663-9812 
856 4 1 |u https://doaj.org/article/cc8c33051a7e4ea7a5b1ef9b1c8389d0  |z Connect to this object online.