Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study

AbstractObjectives The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.Methods From January 2...

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Main Authors: Shiya Huang (Author), Xueqian Huang (Author), Xiaohui Wen (Author), Xuehong Liu (Author), Hongxia Ma (Author), Linling Xie (Author), Yishu Wang (Author), Shanjia Liu (Author), Yongge Guan (Author), Kunyin Li (Author)
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Published: Taylor & Francis Group, 2024-12-01T00:00:00Z.
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001 doaj_ccad501a9f5948c98c8497da7134fc9a
042 |a dc 
100 1 0 |a Shiya Huang  |e author 
700 1 0 |a Xueqian Huang  |e author 
700 1 0 |a Xiaohui Wen  |e author 
700 1 0 |a Xuehong Liu  |e author 
700 1 0 |a Hongxia Ma  |e author 
700 1 0 |a Linling Xie  |e author 
700 1 0 |a Yishu Wang  |e author 
700 1 0 |a Shanjia Liu  |e author 
700 1 0 |a Yongge Guan  |e author 
700 1 0 |a Kunyin Li  |e author 
245 0 0 |a Enhanced glycolysis in the myometrium with ectopic endometrium of patients with adenomyosis: a preliminary study 
260 |b Taylor & Francis Group,   |c 2024-12-01T00:00:00Z. 
500 |a 10.1080/09513590.2024.2332411 
500 |a 1473-0766 
500 |a 0951-3590 
520 |a AbstractObjectives The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.Methods From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.Results Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.Conclusions We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis. 
546 |a EN 
690 |a Adenomyosis 
690 |a myometrium with ectopic endometrium 
690 |a glycolysis 
690 |a glycolytic enzymes 
690 |a lactate 
690 |a Gynecology and obstetrics 
690 |a RG1-991 
690 |a Diseases of the endocrine glands. Clinical endocrinology 
690 |a RC648-665 
655 7 |a article  |2 local 
786 0 |n Gynecological Endocrinology, Vol 40, Iss 1 (2024) 
787 0 |n https://www.tandfonline.com/doi/10.1080/09513590.2024.2332411 
787 0 |n https://doaj.org/toc/0951-3590 
787 0 |n https://doaj.org/toc/1473-0766 
856 4 1 |u https://doaj.org/article/ccad501a9f5948c98c8497da7134fc9a  |z Connect to this object online.