Development of a DNA Vaccine for Melanoma Metastasis by Inhalation Based on an Analysis of Transgene Expression Characteristics of Naked pDNA and a Ternary Complex in Mouse Lung Tissues

• Main Authors: Yukinobu Kodama (Author), Mikiro Nakashima (Author), Tadayuki Nagahara (Author), Natsuko Oyama (Author), Junya Hashizume (Author), Hiroo Nakagawa (Author), Hitomi Harasawa (Author), Takahiro Muro (Author), Tomoaki Kurosaki (Author), Chikamasa Yamashita (Author), Mitsuru Hashida (Author), Takashi Kitahara (Author), Hitoshi Sasaki (Author), Shigeru Kawakami (Author), Tadahiro Nakamura (Author)
• Format: Book
• Published: MDPI AG, 2020-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The present study investigated a pulmonary delivery system of plasmid DNA (pDNA) and its application to melanoma DNA vaccines. pCMV-Luc, pEGFP-C1, and pZsGreen were used as a model pDNA to evaluate transfection efficacy after inhalation in mice. Naked pDNA and a ternary complex, consisting of pDNA,...