Spectrophotometric Determination of Bromhexine Hydrochloride by Azo-Dye Formation Reaction

• Main Authors: Nabeel Othman (Author), Shilan Omer (Author)
• Format: Book
• Published: College of Education for Pure Sciences, 2009-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1812-125X; 2664-2530; 10.33899/edusj.2009.57438
• Keywords: spectrophotometric determination; bromhexine hydrochloride; azo-dye formation reaction; Education; L; Science (General); Q1-390
• Source: مجلة التربية والعلم, Vol 22, Iss 1, Pp 19-31 (2009)

ABSTRACT<br /> A spectrophotometric method for the assay of trace amounts of bromhexine-HCl based on the reaction of bromhexine-HCl with nitrite ion to form the corresponding diazonium salt followed by coupling reaction with pyrogallol in a acidic medium to form a stable and a soluble yellow azo dye with maximum absorption at 387 nm. Beer's law is obeyed in the concentration range of 20-280g of bromhexine-HCl in a final volume of 25 ml (0.8- 11.2 ppm) with a molar absorptivity of 1.031104 L.mol-1.cm-1, a relative error of -0.36 to +0.72 % and <br /> a relative standard deviation of 1.07 to 1.72 %, depending on the concentration level of bromhexine-HCl. The method has been successfully applied for the assay of bromhexine-HCl in various pharmaceutical preparations.