Diagnostic effectiveness of anti-citrullinated peptides antibodies of second and third generations for rheumatoid arthritis
<p><strong>Introduction:</strong> Rheumatoid arthritis is an autoimmune disease characterized by the presence of antibodies against citrullinated peptides, which are one of the indicators for the diagnosis of a disease. The usefulness of different methods for the determination of...
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Universidad de Ciencias Médicas de La Habana,
2018-08-01T00:00:00Z.
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| Summary: | <p><strong>Introduction:</strong> Rheumatoid arthritis is an autoimmune disease characterized by the presence of antibodies against citrullinated peptides, which are one of the indicators for the diagnosis of a disease. The usefulness of different methods for the determination of these antibodies in the diagnosis of Cuban patients with rheumatoid arthritis is necessary to be established.</p><p><strong>Objective:</strong> To establish the diagnostic effectiveness of the second (anti-CCP2) and third (anti-CCP3) generation assays for the determination these antibodies against citrullinated peptides in Cuban patients with rheumatoid arthritis.</p><p><strong>Material and method:</strong> 101 patients with rheumatoid arthritis, 58 patients with other rheumatic and inflammatory diseases, and 43 healthy persons participated in the study. The diagnostic efficiency of rheumatoid factor (RF), anti-CCP2 and anti-CCP3 antibodies were determined using ELISA test, by calculating sensitivity, specificity, and positive and negative predictive values.</p><p><strong>Results:</strong> The anti-CCP2 assay showed a better balance of sensitivity (48.5 %) and specificity (98.0 %). The lower sensibility was observed for RF (40.3%) when the specificity was set at 98 %. Specificity increased to 100% when anti-CCP2 and RF assays were used. All autoantibodies showed association with C-reactive protein and correlation with erythrocyte sedimentation rate. Only anti-CCP2 antibodies showed no correlation with the DAS28 clinical indicator.</p><p><strong>Conclusions:</strong> Anti-CCP2 antibodies are the ones of greater effectiveness in the diagnosis of Cuban patients with rheumatoid arthritis. RF identification allows to identify seronegative anti-CCP2 patients; therefore, the combination of both immunoassays leads to an improvement in the diagnostic effectiveness. </p><p><strong>Keywords:</strong> Rheumatoid arthritis, cyclic citrullinated peptide, antibodies, anti-CCP2, anti-CCP3, diagnosis.</p> |
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| Item Description: | 1729-519X |