Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity

Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to deter...

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Main Authors: Guizhen Du (Author), Mingming Yu (Author), Lingling Wang (Author), Weiyue Hu (Author), Ling Song (Author), Chuncheng Lu (Author), Xinru Wang (Author)
Format: Book
Published: Hindawi Limited, 2018-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Guizhen Du  |e author 
700 1 0 |a Mingming Yu  |e author 
700 1 0 |a Lingling Wang  |e author 
700 1 0 |a Weiyue Hu  |e author 
700 1 0 |a Ling Song  |e author 
700 1 0 |a Chuncheng Lu  |e author 
700 1 0 |a Xinru Wang  |e author 
245 0 0 |a Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity 
260 |b Hindawi Limited,   |c 2018-01-01T00:00:00Z. 
500 |a 1687-966X 
500 |a 1687-9678 
500 |a 10.1155/2018/8608327 
520 |a Cardiac development is a dynamic process and sensitive to environmental chemicals. Triclosan is widely used as an antibacterial agent and reported to transport across the placenta and affect embryonic development. Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. After TCS treatment, the differentiation process was significantly blocked and spontaneous beating rates of CMs were also decreased. Transcriptome analysis showed the dysregulation of genes involved in cardiogenesis, including GATA4 and TNNT2. Additionally, DNA methylation was also altered by TCS exposure, especially in those regions with GATA motif enrichment. These alterations of transcriptome and DNA methylation were all associated with signaling pathways integral to heart development. Our findings indicate that TCS exposure might cause cardiomyocyte differentiation toxicity and provide the new insights into how environmental factors regulate DNA methylation and gene expressions during heart development. 
546 |a EN 
690 |a Internal medicine 
690 |a RC31-1245 
655 7 |a article  |2 local 
786 0 |n Stem Cells International, Vol 2018 (2018) 
787 0 |n http://dx.doi.org/10.1155/2018/8608327 
787 0 |n https://doaj.org/toc/1687-966X 
787 0 |n https://doaj.org/toc/1687-9678 
856 4 1 |u https://doaj.org/article/ce75f33e451b4115a5b9f3a9d9916efd  |z Connect to this object online.