Anti-inflammatory function of arctiin by inhibiting COX-2 expression via NF-κB pathways

• Main Authors: Lee Chong-Kil (Author), Kwak Jin-Hwan (Author), Kwon Jeunghak (Author), Kim Kwanghee (Author), Han Shinha (Author), Kim Hyunyul (Author), Shin Seulmee (Author), Lee Sungwon (Author), Ha Nam-Joo (Author), Yim Dongsool (Author), Kim Kyungjae (Author)
• Format: Book
• Published: BMC, 2011-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>Arctiin, isolated from <it>Forsythia suspensa </it>has been reported to have anti-inflammatory, anti-oxidant, antibacterial, and antiviral effects <it>in vitro</it>. However, there has been a lack of studies regarding its effects on immunological activity. The aim of this study is to investigate the anti-inflammatory potential and possible mechanisms of arctiin in LPS-induced macrophages.</p> <p>Methods</p> <p>We investigated the mRNA and protein levels of proinflammatory cytokines through RT-PCR and western blot analysis, followed by a FACS analysis for surface molecule changes.</p> <p>Results</p> <p>Arctiin dose dependently decreased the production of NO and proinflammatory cytokines such as IL-1β, IL-6, TNF-α, and PGE₂, and it reduced the gene and protein levels as determined by RT-PCR and western blot analysis, respectively. The expression of co-stimulatory molecules such as B7-1 and B7-2 were also inhibited by arctiin. Furthermore, the activation of the nuclear transcription factor, NF-κB in macrophages was inhibited by arctiin.</p> <p>Conclusion</p> <p>Taken together these results provide evidence of the bioactivity of arctiin in inflammatory diseases and suggest that arctiin may exert anti-inflammatory effect by inhibiting the pro-inflammatory mediators through the inactivation of NF-kB.</p>

Item Description

10.1186/1476-9255-8-16
1476-9255