Co-Delivery of Berberine Chloride and Tariquidar in Nanoliposomes Enhanced Intracellular Berberine Chloride in a Doxorubicin-Resistant K562 Cell Line Due to P-gp Overexpression
The MDR phenomenon has become a major obstacle in the treatment of cancers, and among the strategies to reverse it, the inhibition of P-gp function and expression is essential to increase for effective anticancer drugs. In the present paper, the co-delivery of berberine chloride and tariquidar loade...
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MDPI AG,
2021-02-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_cf3e5bb4f84f430f82ea2f8a05dc1d5b | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Giulia Vanti |e author |
| 700 | 1 | 0 | |a Marcella Coronnello |e author |
| 700 | 1 | 0 | |a Daniele Bani |e author |
| 700 | 1 | 0 | |a Antonella Mannini |e author |
| 700 | 1 | 0 | |a Maria Camilla Bergonzi |e author |
| 700 | 1 | 0 | |a Anna Rita Bilia |e author |
| 245 | 0 | 0 | |a Co-Delivery of Berberine Chloride and Tariquidar in Nanoliposomes Enhanced Intracellular Berberine Chloride in a Doxorubicin-Resistant K562 Cell Line Due to P-gp Overexpression |
| 260 | |b MDPI AG, |c 2021-02-01T00:00:00Z. | ||
| 500 | |a 10.3390/pharmaceutics13030306 | ||
| 500 | |a 1999-4923 | ||
| 520 | |a The MDR phenomenon has become a major obstacle in the treatment of cancers, and among the strategies to reverse it, the inhibition of P-gp function and expression is essential to increase for effective anticancer drugs. In the present paper, the co-delivery of berberine chloride and tariquidar loaded nanoliposomes was investigated with the aim of enhancing solubility and improving desired effects for the antineoplastic drug and the P-gp inhibitor. Developed nanoliposomes were loaded with the electron-dense enzyme horseradish peroxidase, and analyzed by TEM to investigate their ability to enter in both K562 and K562/DOXO cell lines. Receptor-mediated endocytosis was evidenced for both cell lines. Nanoliposomes were loaded with tariquidar, berberine chloride, or both, maintaining chemical and physical characteristics-i.e., size, homogeneity, and encapsulation efficiency-and high suitability for parenteral administration. Tariquidar was able to reverse the MDR in the K562/DOXO cell line. Tariquidar- and berberine chloride-loaded nanoliposomes showed a significant increase of berberine chloride accumulation in tumor cells, which could be correlated with resensitization of the resistant cells to the antitumor agent. These results suggest that the co-delivery of the P-gp inhibitor, tariquidar, and the cytotoxicity inducer, berberine chloride, looks like a promising approach to overcome the MDR. | ||
| 546 | |a EN | ||
| 690 | |a MDR | ||
| 690 | |a berberine chloride | ||
| 690 | |a tariquidar | ||
| 690 | |a nanoliposomes | ||
| 690 | |a endocytosis | ||
| 690 | |a uptake | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Pharmaceutics, Vol 13, Iss 3, p 306 (2021) | |
| 787 | 0 | |n https://www.mdpi.com/1999-4923/13/3/306 | |
| 787 | 0 | |n https://doaj.org/toc/1999-4923 | |
| 856 | 4 | 1 | |u https://doaj.org/article/cf3e5bb4f84f430f82ea2f8a05dc1d5b |z Connect to this object online. |