The diagnostic accuracy of non-invasive fetal RhD genotyping by using cell-free fetal DNA in maternal plasma
Introduction: The non-invasive prenataldiagnosis of the fetus RhD genotype in RhD incompatibility has a crucial rolein the prevention of increased anti-D immunoglobulin therapy for haemolyticdiseases in pregnant women carrying RhD negative fetus. It was aimed to detectfetal RhD genotyping by using m...
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| Main Authors: | , , , |
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| Format: | Book |
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SERNEV,
2019-03-01T00:00:00Z.
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| Summary: | Introduction: The non-invasive prenataldiagnosis of the fetus RhD genotype in RhD incompatibility has a crucial rolein the prevention of increased anti-D immunoglobulin therapy for haemolyticdiseases in pregnant women carrying RhD negative fetus. It was aimed to detectfetal RhD genotyping by using maternal circulating cell-free DNA in the currentstudy.Methods: Maternal blood samples werecollected in different trimester of pregnancies (12-40 weeks) in 12 D-negativemothers. Cell-free fetal DNA was extracted from 2 ml of maternal plasma by anconventional DNA isolation technique (Qiagen, Hilden, Germany) and real-timePCR was performed for genotyping target RhD exons 7 and 10 and GLO genes.Postnatal serological evaluations were performed and the results wereconfirmed.Results: 6 cases (50 %)were determined D positive and 6 cases (50 %) were determined D negative. Allresults were also confirmed after birth serologically.Conclusions: In conclusion,the current results showed us the non-invasive target RhD genotyping from cellfree fetal DNA from maternal plasma samples have a diagnostic accuracy in RhDincompatibility pregnancies. |
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| Item Description: | 2459-1505 10.22391/fppc.418647 |