Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC-MS/MS

• Main Authors: Jing Wang (Author), Ji-Ye Sun (Author), Chun-Jie Sha (Author), Yu-Feng Shao (Author), Yan-Hong Liu (Author), You-Xin Li (Author), Zhen-Wen Duan (Author), Wan-Hui Liu (Author)
• Format: Book
• Published: Elsevier, 2015-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...