Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC-MS/MS
A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
| Published: |
Elsevier,
2015-12-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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