HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content

• Main Authors: Heba A.S. El-Nashar (Author), Omayma A. Eldahshan (Author), Abdel Nasser B. Singab (Author)
• Format: Book
• Published: Ain Shams University, 2017-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: https://dx.doi.org/10.21608/aps.2017.10356; 2356-8380; 2356-8399
• Keywords: HPLC; Standardization; quantification; Gallic acid; Acrocarpus fraxinifolius; linearity; Accuracy; Therapeutics. Pharmacology; RM1-950
• Source: Archives of Pharmaceutical Sciences Ain Shams University, Vol 1, Iss 1, Pp 5-8 (2017)

High-Performance Liquid Chromatography (HPLC) was developed for the standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius Weight & Arn leaves. Linearity was observed in the range of 0.4-2 mg/mL with a correlation coefficient (r2) of 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0054 mg/mL and 0.0163 mg/mL, respectively indicating the sensitivity of the applied method. Recovery values of 100.377 % indicate the best accuracy of the method. Gallic acid content was quantified as 168.75 ± 1.05 mg/g methanolic extract. This developed method was simple, accurate and precise to be used as a reference standard method for determination of gallic acid content in the plant extract