Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins
The effects of organic modifiers-alcohols (methanol, isopropanol), acetonitrile, and tetrahydrofuran-and β-cyclodextrins in capillary zone electrophoresis were investigated using a test mixture containing five biogenic amines important from the human health point of view-serotonin, dopamine, adrenal...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Book |
| Published: |
Sciendo,
2022-08-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_d2599c78d77e45d8af70e575d2fb6f31 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Matuskova M. |e author |
| 700 | 1 | 0 | |a Cizmarova I. |e author |
| 700 | 1 | 0 | |a Chalova P. |e author |
| 700 | 1 | 0 | |a Stefanik O. |e author |
| 700 | 1 | 0 | |a Horniakova A. |e author |
| 700 | 1 | 0 | |a Mikus P. |e author |
| 700 | 1 | 0 | |a Piestansky J. |e author |
| 245 | 0 | 0 | |a Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| 260 | |b Sciendo, |c 2022-08-01T00:00:00Z. | ||
| 500 | |a 2453-6725 | ||
| 500 | |a 10.2478/afpuc-2022-0012 | ||
| 520 | |a The effects of organic modifiers-alcohols (methanol, isopropanol), acetonitrile, and tetrahydrofuran-and β-cyclodextrins in capillary zone electrophoresis were investigated using a test mixture containing five biogenic amines important from the human health point of view-serotonin, dopamine, adrenaline, noradrenaline, and tyramine-and two B vitamins-thiamine and pyridoxine. The simultaneous addition of tetrahydrofuran and isopropanol was found to improve the resolution of determined analytes and enable effective separation of analytes with very similar electromigration characteristics migrating as one peak (dopamine and serotonin). The developed and optimised separation method based on capillary zone electrophoresis and ultraviolet detection was capable to achieve detection limits at the concentration level in the range of 0.15 to 1.25 μg/mL−1. The developed method was also characterised by other favourable validation parameters, such as linearity (r2 > 0.99), accuracy (82.9-117.8% for the intraday measurements, and 87.6-119.2% for the interday measurements), and precision (intraday relative standard deviation in the range of 0.4-15.5%, interday relative standard deviation in the range of 0.9-18.3%). The method was finally applied to investigate the stability of the analytes in model water matrix samples under various storage conditions. | ||
| 546 | |a EN | ||
| 690 | |a biogenic amines | ||
| 690 | |a thiamine | ||
| 690 | |a pyridoxine | ||
| 690 | |a capillary zone electrophoresis | ||
| 690 | |a cyclodextrins | ||
| 690 | |a organic solvents | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n European Pharmaceutical Journal, Vol 69, Iss 1, Pp 43-53 (2022) | |
| 787 | 0 | |n https://doi.org/10.2478/afpuc-2022-0012 | |
| 787 | 0 | |n https://doaj.org/toc/2453-6725 | |
| 856 | 4 | 1 | |u https://doaj.org/article/d2599c78d77e45d8af70e575d2fb6f31 |z Connect to this object online. |