Use of a Baculovirus-Mammalian Cell Expression-System for Expression of Drug-Metabolizing Enzymes: Optimization of Infection With a Focus on Cytochrome P450 3A4

• Main Authors: Yuu Miyauchi (Author), Akane Kimura (Author), Madoka Sawai (Author), Keiko Fujimoto (Author), Yuko Hirota (Author), Yoshitaka Tanaka (Author), Shinji Takechi (Author), Peter I. Mackenzie (Author), Yuji Ishii (Author)
• Format: Book
• Published: Frontiers Media S.A., 2022-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Heterologous expression systems are important for analyzing the effects of genetic factors including single nucleotide polymorphisms on the functions of drug-metabolizing enzymes. In this study, we focused on a baculovirus-mammalian cell (Bac-Mam) expression system as a safer and more efficient appr...