Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ

• Main Authors: Nurdiana Dewi (Author), Ahmad Syaify (Author), Ivan Arie Wahyudi (Author)
• Format: Book
• Published: Universitas Airlangga, 2013-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Amelogenin is a major protein constituent of the developing enamel matrix that is critical for enamel formation. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Mutations of amelogenin cause hypoplastic enamel phenotypes. Previous research found that infant of diabetic mother has higher </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>risk for having enamel hypoplasia. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to determine the effect of gestational diabetes mellitus on the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>expression of amelogenin in Wistar rats offspring tooth germ. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: S</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ixteen female Wistar rats, aged 2.5-3 months, body weight </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>150-200 g were used in this study, Wistar rats were mated and divided into two groups and treated on day 0 of pregnancy. Group A was </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>DM group, consisting of 8 rats, induced by streptozotocin (STZ) injection 40 mg/kg BW. Group B was control group, consisting of 8 </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>rats received citrate buffer injection. Thirty-two rat pups were decapitated on day 5. Immunohistochemical procedures were performed </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>on molar tooth germ of the mandibular rat pups using antibody anti-AMELX to determine the expression of amelogenin. Examination </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>carried out on the images using ImageJ software. All data were then statistically analyzed by Mann Whitney test. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Results: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>There was no </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>significant difference in the expression of amelogenin in the DM group and control group (p>0.05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Gestational diabetes </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>mellitus did not affect the expression of amelogenin in rat offspring tooth germ. Further study is needed to examine the pattern of </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>amelogenin expression with measurement of glucose levels of rat pups.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Latar belakang: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Amelogenin merupakan protein terbanyak pada matriks email yang berperan penting dalam pembentukan email. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Mutasi pada amelogenin dapat menyebabkan email menjadi hipoplastik. Penelitian sebelumnya menunjukkan bahwa anak yang </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dilahirkan oleh ibu pengidap diabetes memiliki resiko lebih tinggi untuk mengalami hipoplasia email. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Tujuan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Penelitian ini bertujuan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>untuk meneliti pengaruh diabetes mellitus gestasional terhadap ekspresi amelogenin pada benih gigi anak tikus Wistar. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Metode: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Enam belas ekor tikus Wistar betina, umur 2,5-3 bulan, berat badan 150-200 g digunakan dalam penelitian ini, dikawinkan kemudian </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dibagi menjadi dua kelompok dan diberi perlakuan pada kehamilan hari ke-0. Kelompok A merupakan kelompok diabetes mellitus, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>terdiri atas 8 ekor tikus diberi perlakuan diabetes mellitus dengan injeksi streptozotocin (STZ) 40 mg/kg BB. Kelompok B merupakan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kelompok kontrol, terdiri atas 8 ekor tikus diberi injeksi buffer sitrat. Tiga puluh dua anak tikus yang lahir didekapitasi pada hari ke- </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>5. Dilakukan prosedur imunohistokimia pada benih gigi molar rahang bawah anak tikus menggunakan antibodi anti-AMELX untuk </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>mengetahui ekspresi amelogenin.
Pemeriksaan dilakukan pada hasil foto menggunakan software ImageJ. Hasil kemudian dianalisa </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>menggunakan uji Mann Whitney. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Hasil: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Tidak terdapat perbedaan yang bemakna pada ekspresi amelogenin kelompok kontrol dan </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>kelompok diabetes mellitus (p>0,05). </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Simpulan: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Diabetes mellitus gestasional tidak mempengaruhi ekspresi amelogenin pada benih </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>gigi anak tikus. Diperlukan penelitian lebih lanjut untuk mengetahui pola ekspresi amelogenin dengan pengukuran kadar glukosa </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>darah anak tikus.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></span></p>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v46.i3.p135-139