Chromatographic Techniques and Pharmacological Analysis as a Quality Control Strategy for <i>Serjania triquetra</i> a Traditional Medicinal Plant
<i>Serjania triquetra</i> is a medicinal plant widely used in traditional medicine for the treatment of urinary tract diseases, renal affections, and its complications. The population can buy this plant in folk markets as a raw material mixed with several herbal remedies or as a health s...
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Main Authors: | , , , , , , , , , |
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Format: | Book |
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MDPI AG,
2022-10-01T00:00:00Z.
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Summary: | <i>Serjania triquetra</i> is a medicinal plant widely used in traditional medicine for the treatment of urinary tract diseases, renal affections, and its complications. The population can buy this plant in folk markets as a raw material mixed with several herbal remedies or as a health supplement. On the market, two commercial presentations were found for the vegetal material; one had a bulk appearance and the other was marketed wrapped in cellophane bags (HE<i>St</i>-2, HE<i>St</i>-3). Nevertheless, the plant has not been exhaustively investigated and quality control techniques have not been developed. This research aimed to realize a phytochemical study using an authentic, freshly collected sample as a reference for <i>S. triquetra</i> (HE<i>St</i>-1), using the compounds identified. A method for the determination of preliminary chromatographic fingerprinting was developed. Additionally, the vasorelaxant effect from three samples was evaluated with ex vivo rat models. Thus, three hydroalcoholic extracts (HE<i>St</i>-1, HE<i>St</i>-2, and HE<i>St</i>-3) were prepared by maceration. A total of nine compounds were fully identified from HE<i>St</i>-1 after the extract was subjected to open-column chromatography. Seven metabolites were detected by gas chromatography, while ursolic acid (UA) and allantoin were isolated and identified using UPLC-MS and NMR, respectively. Three extracts were analyzed for their chromatographic fingerprint by UPLC-MS. Biological activity was explored by ex vivo rat aorta ring model to evaluate vasorelaxant activity. All extracts showed a vasorelaxant effect in a concentration-dependent and endothelium-dependent manner. <i>S. triquetra</i> vascular activity may be attributed to UA and allantoin compounds previously described in the literature for this activity. |
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Item Description: | 10.3390/ph15101289 1424-8247 |