The role of liquid smoke coconut shell in the proliferation phase of an oral traumatic ulcer

• Main Authors: Nurina Febriyanti Ayuningtyas (Author), Meircurius Dwi Condro Surboyo (Author), Diah Savitri Ernawati (Author), Adiastuti Endah Parmadiati (Author), Hening Tuti Hendarti (Author), Fatma Yasmin Mahdani (Author), Saka Winias (Author), Fitriatuz Zakia (Author), Indira Arella Harianto (Author)
• Format: Book
• Published: GarVal Editorial Ltda., 2020-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Context: Liquid smoke coconut shell (LS-CS) contains phenolic compounds that are able to promote wound healing by interfering with the inflammation phase of wound healing. The homeostatic mechanism that decreases the inflammation releases a growth factor that enhances proliferation by increasing fibroblast growth factor-2 (FGF-2), vascular endothelial growth factor (VEGF) expression and fibroblast proliferation. Aims: To analyze the role of LS-CS in the proliferation phase of oral traumatic ulcer healing by analyzing fibroblast numbers and FGF-2 and VEGF expressions. Methods: Oral traumatic ulcers were induced in diabetes mellitus Wistar rats via an alloxan injection. A traumatic ulcer as long as 10 mm was made in the inferior fornix incisive labial area. The oral traumatic ulcer was then topically treated with LS-CS using a dose of 20 μL/ 20 g body weight once daily for three, five and seven days. The fibroblast number of the oral traumatic ulcers' tissue was then analyzed using hematoxylin-eosin, and the FGF-2 and VEGF expression were analyzed via immunohistochemistry staining. The difference in fibroblast numbers and FGF-2 and VEGF expressions were analyzed by one-way ANOVA and post hoc tests with a significance of p<0.05. Results: The LS-CS affected fibroblast numbers (p=0.001) and FGF-2 expression (p=0.000) after topical treatment for seven days. There was a significant difference in the expression of VEGF after topical treatment with LS-CS for five days compared to the control group (p=0.000) and benzydamine hydrochloride (p=0.005). The topical treatment of LS-CS for seven days affected the expression of VEGF compared to the control group (p=0.000) and benzydamine hydrochloride (p=0.019). Conclusions: LS-CS could improve the healing of oral traumatic ulcers by increasing fibroblast numbers and FGF-2 and VEGF expression after seven days of treatment.

Item Description

0719-4250