Inflammatory Response to Cobalt-Chromium Alloys Fabricated With Different Techniques

• Main Authors: Maria Kassapidou (Author), Victoria Franke Stenport (Author), Carina B. Johansson (Author), Anna-Karin Östberg (Author), Petra Hammarström Johansson (Author), Lars Hjalmarsson (Author)
• Format: Book
• Published: Stilus Optimus, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.5037/jomr.2021.12403; 2029-283X
• Keywords: cobalt-chromium alloys; cytokine; dental casting technique; inflammation; prosthodontics; titanium; Dentistry; RK1-715
• Source: eJournal of Oral Maxillofacial Research, Vol 12, Iss 4, p e3 (2021)

Objectives: To explore the in vitro cytokine expression of human peripheral blood mononuclear cells exposed to cobalt-chromium alloys, manufactured with different techniques, in comparison with commercially pure titanium grade 4 and titanium alloy grade 23. Material and Methods: Peripheral blood mononuclear cells (PBMC) were collected from 10 healthy blood donors and exposed to machine-ground coin-shaped: (a) cobalt-chromium (Co-Cr) specimens (n = 5) manufactured by four techniques, i.e. cast, milled, laser melted and presintered milled; (b) commercially pure titanium grade 4; and (c) titanium alloy grade 23. The cells were cultured for 4, 24 and 72 hours followed by investigations of pro- and anti-inflammatory cytokine release using Bio-Plex Pro™. Results: In general, the PBMC produced significantly more cytokines when exposed to the cast and presintered milled Co-Cr materials compared to laser melted, milled Co-Cr and titanium materials. Conclusions: Within the limitation of the present study, it may be suggested that cast and presintered milled cobalt-chromium alloys provoke a stronger inflammatory response compared to milled and laser melted cobalt-chromium alloys and titanium materials.