Changes in the contents of four active diterpenoids at different growth stages in <it>Andrographis paniculata</it> (Burm.f.) Nees (<it>Chuanxinlian</it>)

• Main Authors: Pholphana Nanthanit (Author), Rangkadilok Nuchanart (Author), Saehun Jittra (Author), Ritruechai Supachai (Author), Satayavivad Jutamaad (Author)
• Format: Book
• Published: BMC, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>The therapeutic activities of <it>Andrographis paniculata</it> are attributed to four major active diterpenoids: andrographolide (AP₁), 14-deoxy-11,12-didehydroandrographolide (AP₃), neoandrographolide (AP₄), and 14-deoxyandrographolide (AP₆). This study aims to quantify the four active diterpenoids in various plant organs of <it>A. paniculata</it> at different growth stages in greenhouse and field experiments, with a developed HPLC-diode array detector (HPLC-DAD) method for simultaneous determination of these diterpenoids.</p> <p>Methods</p> <p>Plants were grown in greenhouse and in field conditions, harvested at different growth stages, and separated into different organs for determination of the four active diterpenoids by an HPLC-DAD method.</p> <p>Results</p> <p>The most abundant diterpenoid was AP₆ between seedling and vegetative stages in the greenhouse experiment (13.38 to 23.71 mg/g in 2006 and 10.67 to 24.54 mg/g in 2007). High levels of AP₆ were also detected in leaves at the transfer stage in the greenhouse experiment (36.05 ± 0.69 mg/g) and field experiment (30.59 ± 1.39 mg/g). The levels of AP₆ then decreased as plants matured. The highest content of AP₄ was in cotyledons (16.65 ± 4.48 mg/g) at the transfer stage. The highest contents of AP₁ were detected in leaves at seed-forming stage in greenhouse experiment (24.72 ± 1.89 mg/g) and vegetative stage in field experiment (43.16 ± 0.92 mg/g). Flowers of <it>A. paniculata</it> contained high levels of AP₁ (21.42 ± 3.74 mg/g). AP₃ and AP₄ were at low levels in leaves at all growth stages.</p> <p>Conclusion</p> <p>In <it>A. paniculata</it>, AP₆ was at the highest level in leaves at transfer stage in both greenhouse and field experiments. AP₁ was at the highest level in leaves at vegetative stage and seed-forming stage in field and greenhouse experiments, respectively. The contents of AP₃ and AP₄ in leaves were low at all growth stages.</p>

Item Description

10.1186/1749-8546-8-2
1749-8546