Evaluation of nitrate reductase assay on Lowenstein-Jensen medium for drug susceptibility testing of Mycobacterium tuberculosis
Introduction: Multidrug-resistant tuberculosis is of increasing public health concern and a rapid and inexpensive drug susceptibility test is needed in resource limited settings. Aim: This study aims to compare the efficacy of drug susceptibility testing (DST) of Mycobacterium tuberculosis using nit...
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| Main Authors: | , , |
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| Format: | Book |
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Wolters Kluwer Medknow Publications,
2019-01-01T00:00:00Z.
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| Summary: | Introduction: Multidrug-resistant tuberculosis is of increasing public health concern and a rapid and inexpensive drug susceptibility test is needed in resource limited settings. Aim: This study aims to compare the efficacy of drug susceptibility testing (DST) of Mycobacterium tuberculosis using nitrate reductase assay (NRA) on solid Lowenstein-Jensen (LJ) medium with proportion method on LJ medium as reference standard. Materials and Methods: From November 2011 to July 2012, 100 isolates of M. tuberculosis were tested for susceptibility to first-line drugs on LJ medium by proportion method and NRA using LJ with potassium nitrate. Results: For various drugs, the sensitivity and specificity by NRA on solid medium were as follows: streptomycin 96.6% and 98.5%; isoniazid 96.87% and 97%; rifampicin 96.3% and 96%; and ethambutol 78.9% and 91.4%, respectively. The concordance for rifampicin and isoniazid between NRA method and LJ proportion method was found to be >95%. Conclusion: NRA was found to be a rapid and sensitive method for DST of M. tuberculosis. |
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| Item Description: | 0971-9903 10.4103/jmgims.jmgims_59_16 |