Antimicrobial activities of the methanol extract and compounds from the twigs of <it>Dorstenia mannii</it> (Moraceae)
<p>Abstract</p> <p>Background</p> <p><it>Dorstenia mannii</it> (Moraceae) is a medicinal herb used traditionally for the treatment of many diseases. In the present study, the methanol extract of <it>D. mannii</it> and nine of its isolated compoun...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Book |
| Published: |
BMC,
2012-06-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p>Abstract</p> <p>Background</p> <p><it>Dorstenia mannii</it> (Moraceae) is a medicinal herb used traditionally for the treatment of many diseases. In the present study, the methanol extract of <it>D. mannii</it> and nine of its isolated compounds<it>,</it> namely dorsmanin A (<b>1</b>), B (<b>2</b>), C (<b>3</b>), D (<b>4</b>), E (<b>6</b>), F (<b>7</b>), G (<b>8</b>) dorsmanin I (<b>9</b>) and 6,8-diprenyleriodictyol (<b>5</b>), were tested for their antimicrobial activities against yeast, Mycobacteria and Gram-negative bacteria.</p> <p>Methods</p> <p>The microplate alamar blue assay (MABA) and the broth microdilution method were used to determine the minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of the above extract and compounds on a panel of bacterial species.</p> <p>Results</p> <p>The results of the MIC determinations demonstrated that the methanol extract as well as compounds <b>3</b> and <b>8</b> were able to prevent the growth of all the fourteen studied microorganisms within the concentration range of 4 to 1024 μg/ml. The lowest MIC value for the methanol extract (64 μg/ml) was obtained on <it>Candida albicans</it>. The lowest value for individual compounds (4 μg/ml) was recorded with compounds <b>3</b> on <it>Pseudomonas aeruginosa</it> PA01 and <b>7</b> on <it>Eschericia coli ATCC</it> strain. The MIC values recorded with compounds <b>3</b> on <it>P. aeruginosa</it> PA01, <b>6</b> on <it>C. albicans,</it><b>7</b> on <it>P. aeruginosa</it> PA01 and <it>K. pneumoniae</it> ATCC strain and <it>C. albicans,</it>and <b>8</b> on <it>P. aeruginosa</it> PA01, PA124, <it>P. stuartii</it>, <it>M. tuberculosis</it> MTCS1 were lower than or equal to those of the reference drugs. MMC values not greater than 1024 μg/ml were recorded on all studied microorganisms with compounds <b>3</b> and <b>8</b>.</p> <p>Conclusion</p> <p>The overall results of the present investigation provided evidence that the crude extract of <it>D. mannii</it> as well as some of its compounds such compounds <b>3</b> and <b>8</b> could be a potential source of natural antimicrobial products.</p> |
|---|---|
| Item Description: | 10.1186/1472-6882-12-83 1472-6882 |