Entropies of thymocytogram, splenocytogram, immunocytogram and leukocytogram in rats are regulated by sex and the neuroendocrine parameters while regulates immune parameters

• Main Authors: Volodymyra Bilas (Author), Oleksandr Popadynets' (Author), Igor-Severyn Flyunt (Author), Nadiya Sydoruk (Author), Nataliya Badiuk (Author), Serhiy Gushcha (Author), Walery Zukow (Author), Anatoliy Gozhenko (Author), Igor Popovych (Author)
• Format: Book
• Published: Kazimierz Wielki University, 2020-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p class="MsoNormal" style="text-align: justify; text-justify: inter-ideograph;" align="justify"><strong><span style="font-family: 'Times New Roman';">Background.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> We have previously shown that the Entropy </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(E) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of the relative spectral power density </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(SPD) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of the EEG rhythms is its quite relevant parameter. We </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">also </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">found significant </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">relationship</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">s between the SPD E of individual EEG loci and the E of the Leukocytogram </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(LCG) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">and Immunocytogram </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(ICG) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of the blood, as well as with their individual components</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> The </span><strong><span style="font-family: 'Times New Roman';">purpose</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> of this study was to clarify the </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">relationships between E(s) of these four morpho-functional immune subsystem</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">and </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">sex</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> as well as</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">parameters </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of the neuroendocrine-immune complex in rats</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. </span><strong><span style="font-family: 'Times New Roman';">Materials and methods.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">Experiment was performed on </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">10</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">8 healthy Wistar rats </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(48 male and 60 </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">female</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">) weighing </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">20</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">5</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">-</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">30</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">0 g </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(M±SD=260±26 g) </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">divided into </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">8</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received various balneofactors for 6 days</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> The day after the completion of the drinking</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">/application</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> course in all rats </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">the</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">parameters</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">neuroendocrine</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">-</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">immune</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">complex</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">were</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">registered</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">. </span><strong><span style="font-family: 'Times New Roman';">Results.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> The drinking/application course did not affect the E level of any morpho-functional immune subsystem, the relationships between which are insignificant.
Females have less E TCG than males, whereas sexual dimorphism is insignificant for E SCG and ICG and absent for E LCG.</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">The canonical correlation of mediocre force (R=0,675) between sex index (M=1;F=2) and neuro-endocrine parameters </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">HRV</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">calcitonin</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">parathyroid</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">and</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">mineralocorticoid</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">activities</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">corticosterone</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">plasma</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, 17-</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">KS</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">urine</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> was revealed. Factors linked to female sex upregulates nine immune parameters (R=0,630), while downregulates nine others (R=0,678). The neuroendocrine constellation</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> (</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">testosterone</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">plasma, HRV</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">calcitonin</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">parathyroid</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">and</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">mineralocorticoid</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">activities</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> is associated with the constellation of entropies (R=0,533). Entropy of a single morpho-functional immune subsystem is associated with a certain constellation of immune parameters. In particular, E SCG with 12 </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">R=0,721</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, E TCG with 9 </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">R=0,615</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, E ICG with 8 </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">R=0,586</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">, E LCG with 12 </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">(</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">R=0,631</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">)</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">.
</span><strong><span style="font-family: 'Times New Roman';">Conclusion</span></strong><strong><span style="font-family: 'Times New Roman';">.</span></strong><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">E</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">ntrop</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">ies</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">of </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">thymocytogram, splenocytogram, immunocytogram and leukocytogram</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">in rats </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">are </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">re</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">gu</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">lated </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">by</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> sex </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">and </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">the neuroendocrine</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> factors while regulates </span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;">immune</span><span style="mso-spacerun: 'yes'; font-family: 'Times New Roman'; font-size: 12,0000pt;"> parameters.</span></p>

Item Description

2391-8306
10.12775/JEHS.2020.10.07.031