Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

• Main Authors: Su-Jung Shin (Author), Woocheol Lee (Author), Jae-Il Lee (Author), Seung-Ho Baek (Author), Kee-Yeon Kum (Author), Won-Jun Shon (Author), Kwang-Shik Bae (Author)
• Format: Book
• Published: Korean Academy of Conservative Dentistry, 2011-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.5395/JKACD.2011.36.3.196; 2234-7658; 2234-7666
• Keywords: Dentistry; RK1-715
• Source: Restorative Dentistry & Endodontics, Vol 36, Iss 3, Pp 196-202 (2011)

Objectives The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth. Materials and Methods Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05). Results MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level. Conclusions This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.