<i>Saccharomyces Cerevisiae</i> Var <i>Boulardii</i> CNCM I-1079 Reduces Expression of Genes Involved in Inflammatory Response in Porcine Cells Challenged by Enterotoxigenic <i>E. Coli</i> and Influences Bacterial Communities in an In Vitro Model of the Weaning Piglet Colon
Enterotoxigenic <i>Escherichia coli</i> (ETEC) is the main infectious agent responsible for piglet post-weaning diarrhea with high mortality rates. Antimicrobials represent the current principal strategy for treating ETEC infections in pig farms, but the occurrence of multi-resistant bac...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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MDPI AG,
2021-09-01T00:00:00Z.
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| Summary: | Enterotoxigenic <i>Escherichia coli</i> (ETEC) is the main infectious agent responsible for piglet post-weaning diarrhea with high mortality rates. Antimicrobials represent the current principal strategy for treating ETEC infections in pig farms, but the occurrence of multi-resistant bacterial strains has considerably increased in the last decades. Thus, finding non-antibiotic alternatives becomes a real emergency. In this context, we investigated the effect of a live yeast strain, <i>Saccharomyces cerevisiae</i> var <i>boulardii</i> CNCM I-1079 (SB) in an in vitro model of the weaning piglet colon implemented with a mucus phase (MPigut-IVM) inoculated with ETEC and coupled with an intestinal porcine cell line IPI-2I. We showed that SB was able to modulate the in vitro microbiota through an increase in <i>Bacteroidiaceae</i> and a decrease in <i>Prevotellaceae</i> families. Effluents collected from the SB treated bioreactors were able to mitigate the expression level of genes encoding non-gel forming mucins, tight junction proteins, innate immune pathway, and pro-inflammatory response in IPI-2I cells. Furthermore, SB exerted a significant protective effect against ETEC adhesion on porcine IPEC-J2 intestinal cells in a dose-dependent manner and showed a positive effect on ETEC-challenged IPEC-J2 by lowering expression of genes involved in pro-inflammatory immune responses. Our results showed that the strain SB CNCM I-1079 could prevent microbiota dysbiosis associated with weaning and protect porcine enterocytes from ETEC infections by reducing bacterial adhesion and modulating the inflammatory response. |
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| Item Description: | 10.3390/antibiotics10091101 2079-6382 |