TRYBE®: an Fc-free antibody format with three monovalent targeting arms engineered for long in vivo half-life

• Автори: Emma Davé (Автор), Oliver Durrant (Автор), Neha Dhami (Автор), Joanne Compson (Автор), Janice Broadbridge (Автор), Sophie Archer (Автор), Asher Maroof (Автор), Kevin Whale (Автор), Karelle Menochet (Автор), Pierre Bonnaillie (Автор), Emily Barry (Автор), Gavin Wild (Автор), Claude Peerboom (Автор), Pallavi Bhatta (Автор), Mark Ellis (Автор), Matthew Hinchliffe (Автор), David P. Humphreys (Автор), Sam P. Heywood (Автор)
• Формат: Книга
• Опубліковано: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Предмети: article
• Онлайн доступ: Connect to this object online.

Резюме

ABSTRACTTrYbe® is an Fc-free therapeutic antibody format, capable of engaging up to three targets simultaneously, with long in vivo half-life conferred by albumin binding. This format is shown by small-angle X-ray scattering to be conformationally flexible with favorable 'reach' properties. We demonstrate the format's broad functionality by co-targeting of soluble and cell surface antigens. The benefit of monovalent target binding is illustrated by the lack of formation of large immune complexes when co-targeting multivalent antigens. TrYbes® are manufactured using standard mammalian cell culture and protein A affinity capture processes. TrYbes® have been formulated at high concentrations and have favorable drug-like properties, including stability, solubility, and low viscosity. The unique functionality and inherent developability of the TrYbe® makes it a promising multi-specific antibody fragment format for antibody therapy.

Опис примірника

10.1080/19420862.2022.2160229
1942-0870
1942-0862