Assessment of the low-speed centrifugation concept modified in the release of fibroblast growth factor-2 in Saudi healthy patient

• Egile Nagusiak: Abdulrahman Alshehri (Egilea), Salah Alokaili (Egilea), Sami Shafik (Egilea), Nasser M Assery (Egilea), Naghshbandi Jafar (Egilea), Khalid Alhezaimi (Egilea)
• Formatua: Liburua
• Argitaratua: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.
• Notes: 1658-6816; 10.4103/sjos.SJOralSci_97_19
• Keywords: fibroblast growth factor-2; growth factor release; low-speed centrifugation; platelet; platelet-rich fibrin; Dentistry; RK1-715
• Source: Saudi Journal of Oral Sciences, Vol 7, Iss 3, Pp 169-173 (2020)

Introduction: Alteration of the centrifugation time and protocol may impact the release of platelet-rich fibrin (PRF) scaffolds. The current study purpose was to investigate the released levels of fibroblast growth factor-2 (FGF-2) in standard PRF (S-PRF) and low-speed centrifugation concept known as advanced PRF (A-PRF and A-PRF+). Measurements were done at five times interval over 42 days. The aim was to assess the FGF release and the relation between initial platelet counts and the concentrations of FGF-2 release using the following technique: (1) S-PRF, (2) A-PRF, and (3) A-PRF+. Materials and Methods: Twenty-four blood samples were taken from eight random Saudi Arabian national healthy subjects enrolled in the investigation. Blood samples were processed using S-PRF, A-PRF, and A-PRF+ centrifugation protocols. Protein quantification was performed using enzyme-linked immunosorbent assay at 1, 7, 14, 28, and 42-day intervals. Results and Discussion: A statistically significant difference in the mean of FGF-2 measurement between protocols at the 7th day where both S-PRF and A-PRF were significantly higher than A-PRF+ (P < 0.012). Initial platelets' significant count for S-PRF, A-PRF, and A-PRF+ was on day 1, day 7, and 7th day, respectively. Both protocols S-PRF and A-PRF yielded significantly higher release of the FGF-2 when compared to A-PRF+ in Saudi healthy subjects.