Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela

• Main Authors: Armindo Perozo Mena (Author), Marín Milagros (Author), Maribel Castellano (Author), Eliana Ling Toledo (Author), Daniela Núñez (Author), Messaria Ginestre (Author), Jessica Villasmil (Author), José Bermúdez-González (Author), Rafael Villalobos (Author), Liliana Gómez-Gamboa (Author)
• Format: Book
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales, 2017-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The high incidence of the infectious diseases and the antimicrobial resistance arise represent a public health threat today. The extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are an example of this phenomenon. We determined the ESBL-production in Enterobacteriaceae isolates from a Healthcare Center in Maracaibo, during September 2014 to February 2015. The Kirby-Baüer method was perform to preliminary phenotypic detection of ESBL, according to CLSI guidelines. ESBL-production was confirmed by a double-disk synergy test according to the CLSI standards. To genotypic confirmation, the genes blaCTX-M, blaTEM and blaSHV were amplified by PCR. Fifty-five (n=55) strains were analyzed distributed in Escherichia coli (56.36 %), Klebsiella pneumoniae (21.82 %), Enterobacter cloacae (7.27 %), Proteus mirabilis and Serratia marcescens (5.45 % each one), Salmonella spp. and Morganella morganii (1.82 % each one). The major encoded ESBL was the blaTEM gene (83.63 %); followed by 23.63% of the blaCTX-M gene, and 21.81 % encoded the blaSHV gene. 27.27 % of the isolates produced two or three ESBL simultaneously. These results confirmed the high spread of this resistant mechanism among Enterobacteriaceae-producing infections in our public health institutions, therefore control measures should applied to control and reduce its incidence.

Item Description

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