A preliminary study of the anti-inflammatory and anti-apoptotic effects of crocin against gastric ischemia-reperfusion injury in rats
The aim of the present study was to investigate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats. Thirty-two male rats were randomly divided into sham, I/R, I/R + crocin pretreatment and crocin alone groups. To induce I/R lesions, the cel...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Book |
Published: |
Universidade de São Paulo,
2015-09-01T00:00:00Z.
|
Subjects: | |
Online Access: | Connect to this object online. |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
LEADER | 00000 am a22000003u 4500 | ||
---|---|---|---|
001 | doaj_dbddf9de34dc42cbbc59a75d9a7f8d46 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Seyyed Ali Mard |e author |
700 | 1 | 0 | |a Zahra Nikraftar |e author |
700 | 1 | 0 | |a Yaghoob Farbood |e author |
700 | 1 | 0 | |a Esrafil Mansouri |e author |
245 | 0 | 0 | |a A preliminary study of the anti-inflammatory and anti-apoptotic effects of crocin against gastric ischemia-reperfusion injury in rats |
260 | |b Universidade de São Paulo, |c 2015-09-01T00:00:00Z. | ||
500 | |a 2175-9790 | ||
500 | |a 10.1590/S1984-82502015000300015 | ||
520 | |a The aim of the present study was to investigate the protective effect of crocin on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rats. Thirty-two male rats were randomly divided into sham, I/R, I/R + crocin pretreatment and crocin alone groups. To induce I/R lesions, the celiac artery was clamped for 30 min, and the clamp was then removed to allow reperfusion for 3 h. Crocin-pretreated rats received crocin (15 mg/kg, i.p.) 30 min prior to the induction of I/R injury. Samples of gastric mucosa were collected to quantify the protein expression of caspase-3, an apoptotic factor, and inducible nitric oxide synthase (iNOS), a pro-inflammatory protein, by Western blot. Pretreatment with crocin decreased the total area of gastric lesions and decreased the protein expression levels of caspase-3 and iNOS induced by I/R injury. Our findings showed a protective effect of crocin in gastric mucosa against I/R injury. This effect of crocin was mainly mediated by reducing the protein expression of iNOS and caspase-3. | ||
546 | |a EN | ||
690 | |a Crocina/efeito protetor | ||
690 | |a Mucosa gástrica/lesão/isquemia-reperfusão | ||
690 | |a Lesões gástricas/tratamento/estudo experimental | ||
690 | |a Caspase-3 | ||
690 | |a Óxido nítrico sintase induzível | ||
690 | |a Pharmacy and materia medica | ||
690 | |a RS1-441 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Brazilian Journal of Pharmaceutical Sciences, Vol 51, Iss 3, Pp 637-642 (2015) | |
787 | 0 | |n http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300637&lng=en&tlng=en | |
787 | 0 | |n https://doaj.org/toc/2175-9790 | |
856 | 4 | 1 | |u https://doaj.org/article/dbddf9de34dc42cbbc59a75d9a7f8d46 |z Connect to this object online. |