Antidepressant, neuropharmacological activity and mode of action of theaflavin-3-gallate in in vitro and in vivo models of depression

• Main Authors: Ying Wang (Author), Jing Chen (Author), Jing Wang (Author), Xin Guo (Author), Ning Wang (Author), Jie Yuan (Author)
• Format: Book
• Published: Bangladesh Pharmacological Society, 2018-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3329/bjp.v13i4.37773; 1991-0088
• Keywords: Antidepressant; MTT Assay; Neurite outgrowth Neurotoxicity; Polyphenol; Theaflavin-3-gallate; Therapeutics. Pharmacology; RM1-950
• Source: Bangladesh Journal of Pharmacology, Vol 13, Iss 4 (2018)

Herein the antidepressant-like effect of theaflavin-3-gallate, a plant polyphenolic compound in 1-methyl-4-phenyl pyridinium-induced neuronal injury in N2a cells used as an in vitro model of depression as well as in vivo. The results from MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and lactic dehydrogenase assay revealed that theaflavin-3-gallate exerted a neuroprotective effect in N2a cells. Treatments with mixtures of 1-methyl-4-phenyl pyridinium (20 µM) and four concentration levels of theaflavin-3-gallate increased cell viability from 23.3 to 79.6% while lactic dehydrogenase leakage value decreased from 63.2 to 23.4 U/L. Fura-2-acetoxymethyl ester assay for Ca2+ concentration measurement revealed that different doses of theaflavin-3-gallate or fluoxetine (20 µM) reduced the intracellular Ca2+ overloading in N2a cells induced by 1-methyl-4-phenyl pyridinium. Inverted phase microscopy showed that theaflavin-3-gallate induced neurite outgrowth in 1-methyl-4-phenyl pyridinium-induced neurotoxicity in N2a cells. Theaflavin-3-gallate could significantly reduce the immobility time of mice in both forced swimming test and tail suspension test. Video Clip of Methodology: MTT Assay: 3 min 38 sec Full Screen Alternate