Effectiveness of pineapple and papaya leaf combination for dysmenorrhea pain relief in mice (<i>Mus musculus</i>)

• Main Authors: Heriza Syam (Author), Siti Masitoh (Author), Ukhradiya Magharaniq Safira Purwanto (Author), Hasnah Muzakkiyah (Author), Redhalfi Fadhila (Author), Siska Mulyani (Author)
• Format: Book
• Published: PAGEPress Publications, 2024-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.4081/hls.2024.11968; 2281-7824
• Keywords: Papaya leaves; pineapple fruit; primary dysmenorrhea; reducing pain; Public aspects of medicine; RA1-1270
• Source: Healthcare in Low-resource Settings (2024)

Dysmenorrhea is a common gynecological condition in women, often attributed to excessive prostaglandin production, significantly impacting daily activities. Papaya leaves, known for their medicinal properties, are a chosen herbal remedy, albeit with a bitter taste, necessitating combination with pineapple fruit. This study aimed to assess the effectiveness of a combination of papaya leaf extract and pineapple fruit as an alternative treatment for primary dysmenorrhea. In an experimental research design employing the posttest-only control group, 32 male mice were divided into seven groups, injected with acetic acid as a pain inducer, and their writhing responses were recorded for 45 minutes at 15-minute intervals. Data analysis using the ANOVA test revealed significant differences in the number of writhing responses in mice (p <0.05) compared to the positive control group, followed by the Duncan test. The percentage of analgesic protection was as follows: mefenamic acid (61.01%), pineapple fruit extract (62.78%), papaya leaf extract (63.39%), a combination of pineapple and papaya leaf extracts with a dose ratio of 3:1 (73.21%), 2:2 (47.32%), and 1:3 (37.78%). In conclusion, the combination of pineapple extract and papaya leaves in a dose ratio of 3:1 demonstrated the most effective pain reduction.