Failure of Repeated Electroconvulsive Shock Treatment on 5-HT4-Receptor-Mediated Depolarization Due To Protein Kinase A System in Young Rat Hippocampal CA1 Neurons
We previously demonstrated that repeated electroconvulsive shock (ECS) treatment enhanced serotonin (5-HT)1A- and 5-HT3-receptor-mediated responses in hippocampal CA1 pyramidal neurons. The electrophysiological studies were performed to elucidate the effects of ECS treatment on depolarization, which...
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2004-01-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_ddb4d80b089840e19cc2be8d0487da6c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Kumatoshi Ishihara |e author |
| 700 | 1 | 0 | |a Masashi Sasa |e author |
| 245 | 0 | 0 | |a Failure of Repeated Electroconvulsive Shock Treatment on 5-HT4-Receptor-Mediated Depolarization Due To Protein Kinase A System in Young Rat Hippocampal CA1 Neurons |
| 260 | |b Elsevier, |c 2004-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.fp0030382 | ||
| 520 | |a We previously demonstrated that repeated electroconvulsive shock (ECS) treatment enhanced serotonin (5-HT)1A- and 5-HT3-receptor-mediated responses in hippocampal CA1 pyramidal neurons. The electrophysiological studies were performed to elucidate the effects of ECS treatment on depolarization, which was an additional response induced by 5-HT, and the second messenger system involved in this depolarization of hippocampal CA1 neurons. Bath application of 5-HT (100 μM) induced depolarization of the membrane potential in the presence of 5-HT1A-receptor antagonists. This depolarization was mimicked by 5-HT4-receptor agonists, RS 67506 (1 - 30 μM) and RS 67333 (0.1 - 30 μM), in a concentration-dependent manner. 5-HT- and RS 67333-induced depolarization was attenuated by concomitant application of RS 39604, a 5-HT4-receptor antagonist. H-89, a protein kinase A (PKA) inhibitor, inhibited 5-HT-, RS 67506-, and RS 67333-induced depolarizations, while forskolin (10 μM), an activator of adenylate cyclase, induced depolarization. Furthermore, RS 67333-induced depolarization was not significantly different between hippocampal slices prepared from rats administered ECS once a day for 14 days and those from sham-treated rats. These findings suggest that 5-HT4-receptor-mediated depolarization is caused via the cAMP-PKA system. In addition, repeated ECS-treatment did not modify 5-HT4-receptor functions in contrast to 5-HT1A- and 5-HT3-receptor functions. Keywords:: serotonin (5-HT), 5-HT4 receptor, depolarization, hippocampal CA1 region, electroconvulsive shock | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 95, Iss 3, Pp 329-334 (2004) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319324120 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/ddb4d80b089840e19cc2be8d0487da6c |z Connect to this object online. |