Private selective sweeps identified from next-generation pool-sequencing reveal convergent pathways under selection in two inbred Schistosoma mansoni strains.

BACKGROUND: The trematode flatworms of the genus Schistosoma, the causative agents of schistosomiasis, are among the most prevalent parasites in humans, affecting more than 200 million people worldwide. In this study, we focused on two well-characterized strains of S. mansoni, to explore signatures...

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Main Authors: Julie A J Clément (Author), Eve Toulza (Author), Mathieu Gautier (Author), Hugues Parrinello (Author), David Roquis (Author), Jérôme Boissier (Author), Anne Rognon (Author), Hélène Moné (Author), Gabriel Mouahid (Author), Jérôme Buard (Author), Guillaume Mitta (Author), Christoph Grunau (Author)
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Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Julie A J Clément  |e author 
700 1 0 |a Eve Toulza  |e author 
700 1 0 |a Mathieu Gautier  |e author 
700 1 0 |a Hugues Parrinello  |e author 
700 1 0 |a David Roquis  |e author 
700 1 0 |a Jérôme Boissier  |e author 
700 1 0 |a Anne Rognon  |e author 
700 1 0 |a Hélène Moné  |e author 
700 1 0 |a Gabriel Mouahid  |e author 
700 1 0 |a Jérôme Buard  |e author 
700 1 0 |a Guillaume Mitta  |e author 
700 1 0 |a Christoph Grunau  |e author 
245 0 0 |a Private selective sweeps identified from next-generation pool-sequencing reveal convergent pathways under selection in two inbred Schistosoma mansoni strains. 
260 |b Public Library of Science (PLoS),   |c 2013-01-01T00:00:00Z. 
500 |a 1935-2727 
500 |a 1935-2735 
500 |a 10.1371/journal.pntd.0002591 
520 |a BACKGROUND: The trematode flatworms of the genus Schistosoma, the causative agents of schistosomiasis, are among the most prevalent parasites in humans, affecting more than 200 million people worldwide. In this study, we focused on two well-characterized strains of S. mansoni, to explore signatures of selection. Both strains are highly inbred and exhibit differences in life history traits, in particular in their compatibility with the intermediate host Biomphalaria glabrata. METHODOLOGY/PRINCIPAL FINDINGS: We performed high throughput sequencing of DNA from pools of individuals of each strain using Illumina technology and identified single nucleotide polymorphisms (SNP) and copy number variations (CNV). In total, 708,898 SNPs were identified and roughly 2,000 CNVs. The SNPs revealed low nucleotide diversity (π = 2 × 10(-4)) within each strain and a high differentiation level (Fst = 0.73) between them. Based on a recently developed in-silico approach, we further detected 12 and 19 private (i.e. specific non-overlapping) selective sweeps among the 121 and 151 sweeps found in total for each strain. CONCLUSIONS/SIGNIFICANCE: Functional annotation of transcripts lying in the private selective sweeps revealed specific selection for functions related to parasitic interaction (e.g. cell-cell adhesion or redox reactions). Despite high differentiation between strains, we identified evolutionary convergence of genes related to proteolysis, known as a key virulence factor and a potential target of drug and vaccine development. Our data show that pool-sequencing can be used for the detection of selective sweeps in parasite populations and enables one to identify biological functions under selection. 
546 |a EN 
690 |a Arctic medicine. Tropical medicine 
690 |a RC955-962 
690 |a Public aspects of medicine 
690 |a RA1-1270 
655 7 |a article  |2 local 
786 0 |n PLoS Neglected Tropical Diseases, Vol 7, Iss 12, p e2591 (2013) 
787 0 |n http://europepmc.org/articles/PMC3861164?pdf=render 
787 0 |n https://doaj.org/toc/1935-2727 
787 0 |n https://doaj.org/toc/1935-2735 
856 4 1 |u https://doaj.org/article/de8c8f40f7e24c15978d12fe2e7c0ce4  |z Connect to this object online.