Antibacterial, anti-HIV-1 protease and cytotoxic activities of aqueous ethanolic extracts from <it>Combretum adenogonium</it> Steud. Ex A. Rich (Combretaceae)
<p>Abstract</p> <p>Background</p> <p>Records have shown that <it>Combretum adenogonium</it> Steud. Ex A. Rich (Combretaceae) is used in traditional medicine systems of several tribes in Tanzania. This study focused on the investigation of antibacterial activ...
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BMC,
2012-09-01T00:00:00Z.
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| Summary: | <p>Abstract</p> <p>Background</p> <p>Records have shown that <it>Combretum adenogonium</it> Steud. Ex A. Rich (Combretaceae) is used in traditional medicine systems of several tribes in Tanzania. This study focused on the investigation of antibacterial activity, anti-HIV-1 protease activity, toxicity properties and classes of phytochemicals in extracts from <it>C. adenogonium</it> Steud. Ex A. Rich (Combretaceae) to evaluate potential of these extracts for development as herbal remedies.</p> <p>Methods</p> <p>Dried plant material were ground to fine powder and extracted using 80% aqueous ethanol to afford root, leaf and stem bark extracts. The extracts were assayed for anti-HIV-1 protease activities, antibacterial activities using microdilution methods and cytotoxicity using brine shrimps lethality assay. Screening for major phytochemical classes was carried out using standard chemical tests.</p> <p>Results</p> <p>All extracts exhibited antibacterial activity to at least one of the test bacteria with MIC-values ranging from 0.31-5.0 mg/ml. Two extracts, namely, root and stem bark exhibited anti-HIV-1 PR activity with IC<sub>50</sub> values of 24.7 and 26.5 μg/ml, respectively. Stem bark and leaf extracts showed mild toxicity with LC<sub>50</sub> values of 65.768 μg/ml and 76.965 μg/ml, respectively, whereas roots were relatively non-toxic (LC<sub>50</sub> = 110.042 μg/ml). Phytochemical screening of the extracts indicated presence of flavonoids, terpenoids, alkaloids, tannins, glycosides and saponins.</p> <p>Conclusion</p> <p>These results provide promising baseline information for the potential development of <it>C. adenogonium</it> extracts in treatment of bacterial and HIV/AIDS-related opportunistic infections.</p> |
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| Item Description: | 10.1186/1472-6882-12-163 1472-6882 |