Mesoporous Calcium-Silicate Nanoparticles Loaded with Low-Dose Triton-100+Ag<sup>+</sup> to Achieve Both Enhanced Antibacterial Properties and Low Cytotoxicity for Dentin Disinfection of Human Teeth
Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag<sup>+</sup>) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacteria...
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| Main Authors: | , , |
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| Format: | Book |
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MDPI AG,
2021-09-01T00:00:00Z.
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| Summary: | Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag<sup>+</sup>) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacterial properties and low cytotoxicity for dentin disinfection. The physicochemical property, biocompatibility, infiltration ability into dentinal tubules, anti-bacterial ability against both planktonic <i>Enterococcus</i><i>faecalis</i> (<i>E. faecalis</i>) and its biofilm on dentin, effects on dentin microhardness and in vitro mineralization property were systematically investigated. Results confirmed that the MCSNs and M-AgTX nanoparticles showed typical morphology of mesoporous materials and exhibited sustained release of chemicals with an alkaline pH value over time. M-AgTX also exhibited excellent biocompatibility on MC3T3-E1 cells and could eliminate 100% planktonic <i>E. faecalis</i> after 48-h treatment. On dentin slices, it could enter dentinal tubules by ultrasonic activation and inhibit the growth of <i>E. faecalis</i> on dentin. M-AgTX could completely inactive 28-day <i>E. faecalis</i> biofilm. TEM confirmed the destruction of cell membrane integrity and Ag<sup>+</sup> infiltration into bacteria by M-AgTX. Besides, dentin slices medicated with M-AgTX nanoparticles displayed an increased microhardness. After being immersed in SBF for 7 days, apatite crystals could be observed on the surface of the material tablets. M-AgTX could be developed into a new multifunctional intra-canal medication or bone defect filling material for infected bone defects due to its sustained release profile, low cytotoxicity, infiltration ability, enhanced anti-bacterial and mineralization features. |
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| Item Description: | 10.3390/pharmaceutics13091518 1999-4923 |