Pulmonary abnormalities in mice with paracoccidioidomycosis: a sequential study comparing high resolution computed tomography and pathologic findings.

BACKGROUND: Human paracoccidioidomycosis (PCM) is an endemic fungal disease of pulmonary origin. Follow-up of pulmonary lesions by image studies in an experimental model of PCM has not been previously attempted. This study focuses on defining patterns, topography and intensity of lung lesions in exp...

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Main Authors: Damaris Lopera (Author), Tonny Naranjo (Author), José Miguel Hidalgo (Author), Bernardo Miguel de Oliveira Pascarelli (Author), Jairo Hernando Patiño (Author), Henrique Leonel Lenzi (Author), Angela Restrepo (Author), Luz Elena Cano (Author)
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Published: Public Library of Science (PLoS), 2010-01-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Damaris Lopera  |e author 
700 1 0 |a Tonny Naranjo  |e author 
700 1 0 |a José Miguel Hidalgo  |e author 
700 1 0 |a Bernardo Miguel de Oliveira Pascarelli  |e author 
700 1 0 |a Jairo Hernando Patiño  |e author 
700 1 0 |a Henrique Leonel Lenzi  |e author 
700 1 0 |a Angela Restrepo  |e author 
700 1 0 |a Luz Elena Cano  |e author 
245 0 0 |a Pulmonary abnormalities in mice with paracoccidioidomycosis: a sequential study comparing high resolution computed tomography and pathologic findings. 
260 |b Public Library of Science (PLoS),   |c 2010-01-01T00:00:00Z. 
500 |a 1935-2735 
500 |a 10.1371/journal.pntd.0000726 
520 |a BACKGROUND: Human paracoccidioidomycosis (PCM) is an endemic fungal disease of pulmonary origin. Follow-up of pulmonary lesions by image studies in an experimental model of PCM has not been previously attempted. This study focuses on defining patterns, topography and intensity of lung lesions in experimentally infected PCM mice by means of a comparative analysis between High Resolution Computed Tomography (HRCT) and histopathologic parameters. METHODOLOGY: Male BALB/c mice were intranasally inoculated with 3 x 10(6) Paracoccidioides brasiliensis (Pb) conidia (n = 50) or PBS (n = 50). HRCT was done every four weeks to determine pulmonary lesions, quantify lung density, reconstruct and quantify lung air structure. Lungs were also analyzed by histopathology and histomorphometry. RESULTS: Three different patterns of lesions were evidenced by hrct and histopathology, as follows: nodular-diffuse, confluent and pseudo-tumoral. The lesions were mainly located around the hilus and affected more frequently the left lung. At the 4th week post-challenge HRCT showed that 80% of the Pb-infected mice had peri-bronchial consolidations associated with a significant increase in upper lung density when compared with controls, (-263+/-25 vs. -422+/-10 HU, p<0.001). After the 8th and 12th weeks, consolidation had progressed involving also the middle regions. Histopathology revealed that consolidation as assessed by HRCT was equivalent histologically to a confluent granulomatous reaction, while nodules corresponded to individual compact granulomas. At the 16th week of infection, confluent granulomas formed pseudotumoral masses that obstructed large bronchi. Discrete focal fibrosis was visible gradually around granulomas, but this finding was only evident by histopathology. CONCLUSIONS/SIGNIFICANCE: This study demonstrated that conventional HRCT is a useful tool for evaluation and quantification of pulmonary damage occurring in experimental mouse PCM. The experimental design used decreases the need to sacrifice a large number of animals, and serves to monitor treatment efficacy by means of a more rational approach to the study of human lung disease. 
546 |a EN 
690 |a Arctic medicine. Tropical medicine 
690 |a RC955-962 
690 |a Public aspects of medicine 
690 |a RA1-1270 
655 7 |a article  |2 local 
786 0 |n PLoS Neglected Tropical Diseases, Vol 4, Iss 6, p e726 (2010) 
787 0 |n http://europepmc.org/articles/PMC2894136?pdf=render 
787 0 |n https://doaj.org/toc/1935-2735 
856 4 1 |u https://doaj.org/article/e24d235814b74b959e89a5a52023d4b8  |z Connect to this object online.