Altered ganglioside GD3 in HeLa cells might influence the cytotoxic abilities of NK cells

• Main Authors: Wen-Chi Lee (Author), Wen-Ling Lee (Author), Wen-Yuann Shyong (Author), Lin-Wei Yang (Author), Min-Chun Ko (Author), Chang-Ching Yeh (Author), Shie-Liang Edmond Hsieh (Author), Peng-Hui Wang (Author)
• Format: Book
• Published: Elsevier, 2012-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1028-4559; 10.1016/j.tjog.2012.04.006
• Keywords: cervical cancer; cytotoxicity; gangliosides; natural killer cells; sialidases; Gynecology and obstetrics; RG1-991
• Source: Taiwanese Journal of Obstetrics & Gynecology, Vol 51, Iss 2, Pp 199-205 (2012)

Objective: Previously, we found that altered sialidases in HeLa cells in a natural killer-HeLa (NK-HeLa) coculture system contributed to the decreased cytotoxic ability of NK cells. However, changes that occur in the glycosylation of the HeLa cells in the NK-HeLa coculture system remain unknown. Materials and Methods: An NK-HeLa coculture system was used to examine the changes that occur in the gangliosides of HeLa cells. Results: GD3 expression in HeLa cells was significantly increased in the NK-HeLa coculture system. Exogenous ganglioside GD3 decreased the cytotoxic ability of the NK cells, which could be restored by the addition of the anti-GD3 antibody. Coadministration of GD3 and sialidase further decreased the cytotoxic ability of the NK cells, which could be partially restored by the addition of a sialidase inhibitor (DANA). GD3 expression in HeLa cells also decreased following DANA treatment. Conclusions: This study suggests that interactions between ganglioside GD3 and sialidases in HeLa cells influence the cytotoxic ability of NK cells.