IMMUNOHISTROCHEMISTRY AND FLOW CYTOMETRIC CHARACTERISTICS OF INTRATUMORAL LYMPHOCYTES IN OVARIAN SEROUS ADENOCARCINOMA

• Main Authors: E. V. Zuyeva (Author), S. O. Nikogosyan (Author), V. V. Kuznetsov (Author), A. V. Mozhenkova (Author), N. V. Kokosadze (Author), V. V. Timoshenko (Author), M. Zh. Karibayeva (Author), N. N. Tupitsyn (Author)
• Format: Book
• Published: ABV-press, 2014-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Intratumoral T-lymphocyte subpopulations were studied in 27 patients with ovarian serous adenocarcinoma, by using immunohisto- chemical techniques (n = 27) and three-color flow cytometry (n = 11).The subpopulations of intratumoral lymphocytes infiltrating the tumor parenchyma and stroma differed. The intraepithelial cells were mainly CD8+ T cells (30.7%) and CD56+ lymphocytes (25%), which were indicative of the predominance of NK cells. Stromal infil- tration was primarily presented by mature CD3+ T lymphocytes (33%). This was supported by flow cytometric studies; CD3+ constitut- ed 62-92.6% among the CD45+ intratumoral leukocytes. The CD3+CD8+/CD3+CD4+ ratio was > 2 only in 3 cases. CD25+ cells made up the bulk among the CD3+CD4+ lymphocytes - 30-60% in 6 patients

Item Description

1994-4098
1999-8627
10.17650/1994-4098-2009-0-3-4-117-121