Interactions between DMPC Model Membranes, the Drug Naproxen, and the Saponin <i>β</i>-Aescin

• Main Authors: Pia Hägerbäumer (Author), Friederike Gräbitz-Bräuer (Author), Marco Annegarn (Author), Carina Dargel (Author), Tim Julian Stank (Author), Thomas Bizien (Author), Thomas Hellweg (Author)
• Format: Book
• Published: MDPI AG, 2023-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In this study, the interplay among the phospholipid 1,2-dimyristoyl-<i>sn</i>-glycero-3-phosphocholine (DMPC) as a model membrane, the nonsteroidal anti-inflammatory drug naproxen, and the saponin <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>β</mi></semantics></math></inline-formula>-aescin are investigated. The naproxen amount was fixed to 10 mol%, and the saponin amount varies from 0.0 to 1.0 mol%. Both substances are common ingredients in pharmaceutics; therefore, it is important to obtain deeper knowledge of their impact on lipid membranes. The size and properties of the DMPC model membrane upon naproxen and aescin addition were characterized with differential scanning calorimetry (DSC), small- and wide-angle X-ray scattering (SAXS, WAXS), and photon correlation spectroscopy (PCS) in a temperature-dependent study. The interaction of all substances was dependent on the lipid phase state, which itself depends on the lipid's main phase transition temperature <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mi>T</mi><mi mathvariant="normal">m</mi></msub></semantics></math></inline-formula>. The incorporation of naproxen and aescin distorted the lipid membrane structure and lowers <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mi>T</mi><mi mathvariant="normal">m</mi></msub></semantics></math></inline-formula>. Below <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mi>T</mi><mi mathvariant="normal">m</mi></msub></semantics></math></inline-formula>, the DMPC-naproxen-aescin mixtures showed a vesicle structure, and the insertion of naproxen and aescin influenced neither the lipid chain-chain correlation distance nor the membrane thickness. Above <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><msub><mi>T</mi><mi mathvariant="normal">m</mi></msub></semantics></math></inline-formula>, the insertion of both molecules instead induced the formation of correlated bilayers and a decrease in the chain-chain correlation distance. The presented data clearly confirm the interaction of naproxen and aescin with DMPC model membranes. Moreover, the incorporation of both additives into the model membranes is evidenced.

Item Description

10.3390/pharmaceutics15020379
1999-4923