Hydrogen Peroxide Increases during Endodormancy and Decreases during Budbreak in Grapevine (<i>Vitis vinifera</i> L.) Buds

• Main Authors: Francisco Javier Pérez (Author), Ximena Noriega (Author), Sebastián Rubio (Author)
• Format: Book
• Published: MDPI AG, 2021-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Changes in the level of hydrogen peroxide (H₂O₂) is a good indicator to monitor fluctuations in cellular metabolism and in the stress responses. In this study, the changes in H₂O₂ content during bud endodormancy (ED) and budbreak were analysed in grapevine (<i>Vitis vinifera</i> L.). The results showed a gradual increase in the H₂O₂ content during the development of bud ED, which was mainly due to an increase in the activity of peroxidases (PODs). The maximum H₂O₂ content reached in the grapevine buds coincided with the maximum depth of bud ED. In contrast, during budbreak, the H₂O₂ content decreased. As the plant hormones cytokinin (CK) and auxin play an important role in budbreak and growth resumption in grapevine, the effect of exogenous applications of H₂O₂ on the expression of genes involved in CK and auxin metabolism was analysed. The results showed that H₂O₂ represses the expression of the CK biosynthesis genes <i>VvIPT3a</i> and <i>VvLOG1</i> and induces the expression of the CK-inactivating gene <i>VvCKX3</i>, thus reducing potentially the CK content in the grapevine bud. On the other hand, H₂O₂ induced the expression of the auxin biosynthesis genes <i>VvAMI1</i> and <i>VvYUC3</i> and of the auxin transporter gene <i>VvPIN3</i>, thus increasing potentially the auxin content and auxin transport in grapevine buds. In general, the results suggest that H₂O₂ in grapevine buds is associated with the depth of ED and negatively regulates its budbreak.

Item Description

10.3390/antiox10060873
2076-3921