Effect in vitro of the Phenobarbital on the Activity of the Enzyme Adenosine Triphosphatase (ATPase) of Sodium and Potassium Dependent in Human Placenta.

• Main Authors: María De Jesús Sánchez Bouza (Author), Pedro Sánchez Frenes (Author)
• Format: Book
• Published: Centro Provincial de Información de Ciencias Médicas. Cienfuegos, 2007-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1727-897X
• Keywords: fenobarbital; estabilidad enzimática, placenta; Medicine (General); R5-920; Public aspects of medicine; RA1-1270
• Source: Medisur, Vol 5, Iss 3, Pp 12-17 (2007)

<p><strong>Background: </strong>Most of the drugs can pass to the fetus through the placenta, conditioning alterations of the development and fetal growth. <strong>Objective:</strong> To evaluate the effect in vitro of Phenobarbital on the activity of adenosine triphosphatase of sodium and potassium dependent in human placenta. <strong>Method: </strong>A study in vitro was carried out, in total homogenizes of placentas coming from 30 mothers that were assisted in the Gynecological and Obstetric Educational Provincial Hospital ¨Mariana Grajales¨, in Santa Clara, during the period March-July 1993. Phenobarbital was administered in concentrations of 0,1 mg/ml, 0,15 mg/ml and 0,2 mg/ml, keeping in mind the therapeutic dose to which was prescribed. The analyzed variables were: enzymatic activity and inhibition type. <strong>Results:</strong> The enzymatic activity of the adenosine triphosphatase of sodium and potassium dependent, diminished in a significant way, proportionally with the diminishment of the administered concentration of Phenobarbital. The inhibitory effect of the drug also turned out to be dependent of the concentration, presenting a major inhibition as the dose was increased. <strong>Conclusion:</strong> Phenobarbital produced highly significant decrease in the activity of adenosine triphosphatase of sodium and dependent potassium. This inhibition is of competitive type.</p>