Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements

• Main Authors: Nayara Nery de Oliveira Cunha (Author), Marina Azevedo Junqueira (Author), Leopoldo Cosme-Silva (Author), Laís da Silveira Terra Santos (Author), George Augusto Veloso de Oliveira (Author), Rafael Tobias Moretti Neto (Author), Denismar Alves Nogueira (Author), Maísa Ribeiro Pereira Lima Brigagão (Author), Ana Beatriz da Silveira Moretti (Author)
• Format: Book
• Published: Association of Support to Oral Health Research (APESB), 2021-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.

Item Description

1519-0501
1983-4632