A Single Infusion of Polyethylene Glycol-Coated Superparamagnetic Magnetite Nanoparticles Alters Differently the Expressions of Genes Involved in Iron Metabolism in the Liver and Heart of Rats
This study investigated genotype- and tissue-related differences in the biodistribution of superparamagnetic magnetite (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles (IONs) into the heart and liver of normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats afte...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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MDPI AG,
2023-05-01T00:00:00Z.
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| Summary: | This study investigated genotype- and tissue-related differences in the biodistribution of superparamagnetic magnetite (Fe<sub>3</sub>O<sub>4</sub>) nanoparticles (IONs) into the heart and liver of normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats after a single i.v. infusion of polyethylene glycol-coated IONs (~30 nm, 1mg Fe/kg) 100 min post-infusion. The effects of IONs on the expression of selected genes involved in the regulation of iron metabolism, including <i>Nos</i>, <i>Sod</i> and <i>Gpx4</i>, and their possible regulation by nuclear factor (erythroid-derived 2)-like 2 (NRF2, encoded by <i>Nfe2l2</i>) and iron-regulatory protein (encoded by <i>Irp1</i>) were investigated. In addition, superoxide and nitric oxide (NO) production were determined. Results showed reduced ION incorporations into tissues of SHR compared to WKY and in the hearts compared to the livers. IONs reduced plasma corticosterone levels and NO production in the livers of SHR. Elevated superoxide production was found only in ION-treated WKY. Results also showed differences in the regulation of iron metabolism on the gene level in the heart and liver. In the hearts, gene expressions of <i>Nos2</i>, <i>Nos3</i>, <i>Sod1</i>, <i>Sod2</i>, <i>Fpn</i>, <i>Tf</i>, <i>Dmt1</i> and <i>Fth1</i> correlated with <i>Irp1</i> but not with <i>Nfe2l2,</i> suggesting that their expression is regulated by mainly iron content. In the livers, expressions of <i>Nos2</i>, <i>Nos3</i>, <i>Sod2</i>, <i>Gpx4</i>, and <i>Dmt1</i> correlated with <i>Nfe2l2</i> but not with <i>Irp1</i>, suggesting a predominant effect of oxidative stress and/or NO. |
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| Item Description: | 10.3390/pharmaceutics15051475 1999-4923 |