Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO
Arteriosclerosis obliterans (ASO) of the lower extremities is identified as a kind of cardiovascular disease with aberrant proliferation and apoptosis of vascular smooth muscle cells (VSMCs). Accumulating studies have demonstrated the vital role of Yes1-associated transcriptional regulator (YAP1) in...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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Elsevier,
2021-12-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_e92f3106592d4e27b7f95b51eab9f729 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Xue Zhang |e author |
| 700 | 1 | 0 | |a Peng Wang |e author |
| 700 | 1 | 0 | |a Kai Yuan |e author |
| 700 | 1 | 0 | |a Maoran Li |e author |
| 700 | 1 | 0 | |a Yiting Shen |e author |
| 700 | 1 | 0 | |a Huafa Que |e author |
| 700 | 1 | 0 | |a Yunfei Wang |e author |
| 700 | 1 | 0 | |a Wei Liang |e author |
| 245 | 0 | 0 | |a Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO |
| 260 | |b Elsevier, |c 2021-12-01T00:00:00Z. | ||
| 500 | |a 2162-2531 | ||
| 500 | |a 10.1016/j.omtn.2021.07.026 | ||
| 520 | |a Arteriosclerosis obliterans (ASO) of the lower extremities is identified as a kind of cardiovascular disease with aberrant proliferation and apoptosis of vascular smooth muscle cells (VSMCs). Accumulating studies have demonstrated the vital role of Yes1-associated transcriptional regulator (YAP1) in VSMCs, while its upstream regulatory mechanism in VSMCs in ASO of the lower extremities needs to be further elucidated. Herein, hsa_circ_0024093, a circular RNA (circRNA) from YAP1, was identified to positively regulate the protein level of YAP1 in VSMCs. Functionally, silencing of hsa_circ_0024093 obviously impeded cell proliferation and migration and promoted apoptosis in VSMCs in the in vitro model of ASO of the lower extremities. Mechanistically, it was found that hsa_circ_0024093 could regulate the expression of USP9X, which further induced YAP1 deubiquitination to stabilize YAP1 protein. In depth, it was revealed from mechanism experiments that hsa_circ_0024093 sequestered miR-889-3p or miR-4677-3p to enhance USP9X expression. Further, rescue assays validated that hsa_circ_0024093 regulated the miR-4677-3p/miR-889-3p/USP9X axis to accelerate the proliferation and migration of VSMCs in the in vitro model of ASO of the lower extremities. These findings may provide a novel perspective for better understanding of ASO of the lower extremities. | ||
| 546 | |a EN | ||
| 690 | |a arteriosclerosis obliterans | ||
| 690 | |a lower extremities | ||
| 690 | |a vascular smooth muscle cells | ||
| 690 | |a hsa_circ_0024093 | ||
| 690 | |a YAP1 | ||
| 690 | |a USP9X | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Molecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 511-522 (2021) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S2162253121001943 | |
| 787 | 0 | |n https://doaj.org/toc/2162-2531 | |
| 856 | 4 | 1 | |u https://doaj.org/article/e92f3106592d4e27b7f95b51eab9f729 |z Connect to this object online. |