Silver Nanoparticles Biosynthesized with Spruce Bark Extract-A Molecular Aggregate with Antifungal Activity against <i>Candida</i> Species
Due to their high content of biomolecules, combined with silver's well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
MDPI AG,
2021-10-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Due to their high content of biomolecules, combined with silver's well known antimicrobial potential, silver nanoparticles biosynthesized using spruce bark (AgNP SBEs) demonstrate antibacterial and antioxidant activity, making them a versatile option for developing new antimicrobial agents that might be used for medical treatment or as adjuvants for the classical agents. This study aims to analyze if silver nanoparticles (AgNPs) mediated by spruce bark extract (SBE) and silver salts (AgNP SBE Acetate, AgNP SBE Nitrate) presents antifungal activity against five different <i>Candida</i> spp., synergistic activity with fluconazole, and if they interact with some virulence factors of <i>C. albicans</i>. AgNP SBEs presented MICs (minimum inhibitory concentrations) for all the five tested <i>Candida</i> spp. AgNP SBEs inhibited the growth of <i>C. parapsilosis</i>, <i>C. krusei,</i> and <i>C. guilliermondii,</i> exerted synergistic activity with fluconazole for <i>C. parapsilosis</i> and <i>C. guilliermondii</i>, and inhibited biofilm production for <i>C. albicans</i>, <i>C. auris</i>, and <i>C. guilliermondii</i>. MICs of AgNP SBE Acetate significantly inhibited the production of germ tubes of <i>C. albicans</i>. The expression of <i>C. albicans</i> <i>SAP2</i> gene was down-regulated by the short-time treatment with MICs of AgNP SBE Acetate, while <i>ALS3</i> and <i>HSP70</i> genes were up-regulated by the AgNPs MICs. These results emphasize the potential of using the AgNP SBEs as treatments/adjuvants options, not only against the redundant <i>C. albicans</i> but also for the non-albicans <i>Candida</i> species (which are not as frequently involved in human pathologies, but, sometimes, can be more aggressive). |
|---|---|
| Item Description: | 10.3390/antibiotics10101261 2079-6382 |