MiR-526b-3p enhances sensitivity of head and neck squamous cell carcinoma cells to radiotherapy via suppressing exosomal LAMP3-mediated autophagy

Lysosomal associated membrane protein 3 (LAMP3) has been reported to be a tumour promoter in multiple cancer types by modulating tumour cell autophagy. However, the potential mechanism of LAMP3 in radio-resistance of head and neck squamous cell carcinoma (HNSCC) remains unknown. Therefore, our curre...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Principais autores: Huixiang Lu (Autor), Junnian Zhou (Autor), Xiaojun Li (Autor), Xiaoqin Han (Autor), Shuping Ma (Autor), Chunlan Feng (Autor)
Formato: Livro
Publicado em: Taylor & Francis Group, 2023-12-01T00:00:00Z.
Assuntos:
Acesso em linha:Connect to this object online.
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

MARC

LEADER 00000 am a22000003u 4500
001 doaj_e9f21efab69840afab6475cdcd3d54c1
042 |a dc 
100 1 0 |a Huixiang Lu  |e author 
700 1 0 |a Junnian Zhou  |e author 
700 1 0 |a Xiaojun Li  |e author 
700 1 0 |a Xiaoqin Han  |e author 
700 1 0 |a Shuping Ma  |e author 
700 1 0 |a Chunlan Feng  |e author 
245 0 0 |a MiR-526b-3p enhances sensitivity of head and neck squamous cell carcinoma cells to radiotherapy via suppressing exosomal LAMP3-mediated autophagy 
260 |b Taylor & Francis Group,   |c 2023-12-01T00:00:00Z. 
500 |a 0891-6934 
500 |a 1607-842X 
500 |a 10.1080/08916934.2023.2259125 
520 |a Lysosomal associated membrane protein 3 (LAMP3) has been reported to be a tumour promoter in multiple cancer types by modulating tumour cell autophagy. However, the potential mechanism of LAMP3 in radio-resistance of head and neck squamous cell carcinoma (HNSCC) remains unknown. Therefore, our current study aims to detect the impacts of LAMP3 on the resistance of HNSCC cells to radiotherapy and meanwhile explore its functional mechanism. Through RT-Qpcr examination, LAMP3 expression was identified to be expressed at a significantly high level in irradiation-resistant HNSCC cell lines compared with irradiation-sensitive HNSCC cell lines. Functional assays including CCK-8, colony formation and Transwell assays demonstrated that LAMP3 enhanced the radio-resistance through inducing autophagy to promote HNSCC cell growth. Furthermore, irradiation-resistant HNSCC cells could transfer exosomal LAMP3 to elevate LAMP3 expression in irradiation-sensitive HNSCC cells. Mechanistically, microRNA (miRNA) miR-526b-3p could inhibit LAMP3 expression so as to strengthen sensitivity of HNSCC cells to radiotherapy. In a word, exosomal LAMP3 expression promoted radioresistance of HNSCC cells via inducing autophagy, while this effect could be suppressed by miR-526b-3p in a targeted manner. 
546 |a EN 
690 |a lamp3 
690 |a hnscc 
690 |a radioresistance 
690 |a mir-526b-3p 
690 |a autophagy 
690 |a Internal medicine 
690 |a RC31-1245 
655 7 |a article  |2 local 
786 0 |n Autoimmunity, Vol 56, Iss 1 (2023) 
787 0 |n http://dx.doi.org/10.1080/08916934.2023.2259125 
787 0 |n https://doaj.org/toc/0891-6934 
787 0 |n https://doaj.org/toc/1607-842X 
856 4 1 |u https://doaj.org/article/e9f21efab69840afab6475cdcd3d54c1  |z Connect to this object online.