Local Application of a New Chalconic Derivative (Chalcone T4) Reduces Inflammation and Oxidative Stress in a Periodontitis Model in Rats

• Main Authors: Angelo Constantino Camilli (Author), Mariely Araújo de Godoi (Author), Vitória Bonan Costa (Author), Natalie Aparecida Rodrigues Fernandes (Author), Giovani Cirelli (Author), Larissa Kely Faustino da Silva (Author), Letícia Ribeiro Assis (Author), Luis Octavio Regasini (Author), Morgana Rodrigues Guimarães-Stabili (Author)
• Format: Book
• Published: MDPI AG, 2024-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Chalcones are phenolic compounds with biological properties. This study had the aim to evaluate the effects of topical administration of a new synthetic chalcone, Chalcone T4, in an animal model of periodontitis induced by ligature. Forty rats were distributed in the following experimental groups: negative control (without periodontitis and topical application of distilled water), positive control (periodontitis and topical application of distilled water), chalcone I and II (periodontitis and topical application of 0.6 mg/mL and 1.8 mg/mL, respectively). Chalcone or distilled water was administered into the gingival sulcus of the first molars daily for 10 days, starting with the ligature installation. The following outcomes were evaluated: alveolar bone loss (µCT and methylene blue dye staining), quantification of osteoclasts (histomorphometry), cell infiltrate and collagen content (stereometry), gene expression of mediators (<i>Nfact11, Tnf-α, Mmp-13, iNos, Sod and Nrf2</i>) by (RT-qPCR); expression of BCL-2 and Caspase-1 (immunohistochemistry). Chalcone T4 inhibited bone resorption and prevented collagen matrix degradation. Reduction in the expression of inflammatory markers (<i>Nfact11, Tnf-α, Mmp-13</i>, and Caspase-1), attenuation of oxidative stress (<i>iNOS</i> reduction, and increase in <i>Sod</i>), and pro-apoptotic effect of the compound (BCL-2 reduction), were associated its effects on periodontal tissues. Topical application of Chalcone T4 prevented bone resorption and inflammation, demonstrating potential in the adjunctive treatment of periodontitis.

Item Description

10.3390/antiox13101192
2076-3921