Screening Salvia macrosiphon transcriptome for 4-coumarate CoA ligase enzyme coding genes
<p>4-Coumarate CoA ligase (4CL) is one of the key enzymes in the phenylpropanoid pathway which converts cinnamic acid derivatives to active thioesters. Active thioesters are precursors of a vast number of secondary metabolites. <em>Salvia macrosiphon</em> as a valuable medicinal pl...
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| Main Authors: | , |
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| Format: | Book |
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Shiraz University of Medical Sciences,
2016-08-01T00:00:00Z.
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| Summary: | <p>4-Coumarate CoA ligase (4CL) is one of the key enzymes in the phenylpropanoid pathway which converts cinnamic acid derivatives to active thioesters. Active thioesters are precursors of a vast number of secondary metabolites. <em>Salvia macrosiphon</em> as a valuable medicinal plant grows in Iran, Turkey and Afghanistan. This plant produces pharmacologically active metabolites including rosmarinic acid, flavonoids (apigenin, luteolin), sesquiterpenes and coumarins. Identification of genes encoding <em>4cl</em> provides the opportunity to manipulate the biosynthetic pathways and mediate the carbon flux toward the corresponding metabolites. Mature wild type <em>S. macrosiphon</em> plants were collected in flowering and seed-bearing stages. <em>In vitro</em> cultures of <em>S. macrosiphon </em>were established on Murashige & Skoog (1/2 MS) medium. To increase the possibility of finding <em>4cl</em> isoforms in the transcriptome, cultures were elicited by chitosan. Since the genomic sequence of <em>S. macrosiphon</em> was not available, degenerate and CODEHOP primers were designed based on the identified 4CL protein sequences. Using the CODEHOP primers two isoforms of putative <em>4cl</em> genes have been identified in all organs of the wild type plant. Degenerate primers could only amplify the same genes from roots and seed-containing capsules. This might be due to a higher expression level of the genes in these organs. No <em>4cl</em> isoform have been detected from cultures which might be due to the lower abundance of the transcript at early stages of <em>in vitro</em> plantlets. Phylogenetic analysis showed that the two isoforms of <em>4cl</em> genes from <em>S. macrosiphon </em>and <em>Salvia miltiorrhiza</em> have been evolved from a common ancestor.</p> |
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| Item Description: | 2423-3722 2423-5652 10.1111/tips.v2i3.84 |